FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347332
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG326221997.45728837175398No Hit
TAATGGTG91310.2727844145725611No Hit
TATTGGGG77340.23104968374813134No Hit
TATTGGTA77250.23078081289815291No Hit
AATTGGTG70140.20954001574985692No Hit
TATTGGTC60320.18020321856332147No Hit
TATTGCTG54360.16239799338697208No Hit
TATTGGAG53160.158813048720593No Hit
TATTCGTG49780.14871545457695862No Hit
TATTGGTT46250.13816974235002683No Hit
TTTTGGTG45790.1367955135612482RNA PCR Primer, Index 28 (100% over 8bp)
TATAGGTG45050.13458479768364776RNA PCR Primer, Index 39 (100% over 8bp)
TAGTGGTG38860.11609245811290902No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)