FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3202040
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT563231.758972405091754No Hit
GAAGCAGTGGAACCTT176870.5523666162821201No Hit
GTTCTTCCTAACGTAA163450.5104558344055664No Hit
GATACGTACCGCTCGA153300.4787572922262058No Hit
CGCTTAAGATAATAGG131210.4097700216112228No Hit
CATGGAGGATCGACAT129330.4038987645376073No Hit
TGTCCCATGAGACAAT127860.399307941187493No Hit
AAAGCATGATCTTTGT118520.3701390363643177No Hit
TGAGCGCACAGGACAC111470.34812182233825933No Hit
TGTCGAAACTTCGCAA106590.33288153801951254No Hit
TAATTGCCTGTCTGGC104450.3261982985846523No Hit
GGGGGGGGGGGGGGGG99440.3105520230852831No Hit
GGAAGGACTAGGTAAT85830.2680478694832044No Hit
AATGATGGAAAGCTAA85790.267922949119936No Hit
CATGTCAGAGGTTTGT85410.2667362056688861No Hit
TCGACCTTGTTTCGCC78660.24565589436734084No Hit
ATGGGTACTTGGCTGC64760.20224606813156612No Hit
GCTATGATGACCTGCG62650.19565651896915714No Hit
AAACGACCTAGGGAGG60390.18859851844449163No Hit
TTGCGATGACATTCAG59700.18644364217811146No Hit
AGCCTACCTTAACTCG59630.18622503154239173No Hit
CAGCTCACTGTTTACG59510.18585027045258648No Hit
TGTTCCTCTTTATGGC57130.1784175088381157No Hit
CCTGTTGACCCTAGTA54910.1714844286767186No Hit
CTGCCTTGACCATAAC54600.17051629586138836No Hit
CAATGTGACCAACGCC52790.16486364942349252No Hit
TAACCAGACATCACAC52190.16298984397446628No Hit
CATGCAAACGAAGGCT51880.16202171115913605No Hit
CACAGCTCTGGACTGA51590.16111603852544004No Hit
ATGGGTACTTTCGGGC50710.15836779053353486No Hit
CATGATGTGTCATGTG50660.15821164007944935No Hit
AGCTTGTGACTCACTC50290.15705612671921648No Hit
CTATTCAACCGAACGA49780.15546339208754417No Hit
GTGAGATCTCGATTAC49360.15415172827322582No Hit
TTGTTTGTGATACGCT48700.15209054227929694No Hit
TGGGCCATGCGTTCTC48010.14993566601291675No Hit
ATTAGCGTGGTCGGTC47420.14809309065470763No Hit
ATGCCGCGATGCCACG47220.14746848883836555No Hit
CCGCTATTGTTCGCAA46640.1456571435709735No Hit
CATTAGTCTATTCTGG45830.14312750621478806No Hit
AGAACGCGAGGAATAT45690.14269028494334862No Hit
GCATCTCTGAGCCTAT45570.14231552385354337No Hit
GGGCTGACTAATACAG45170.14106632022085921No Hit
CACTATACTACGCCAC44870.14012941749634608No Hit
GGGTCGTACCGCTCGA44860.14009818740552896No Hit
ATAGGAAACAGACTTC43050.13444554096763314No Hit
AACCAACACCGGAATG42070.13138499206755694No Hit
TTGACGTGACCTCAAA42040.13129130179510562No Hit
GCAGATACTCGGCTCA41960.1310414610685688No Hit
AGCTCGGACCTTAAGC41850.13069793006958064No Hit
CCCAATGCTGGAATGC41830.13063546988794644No Hit
AACGTCCCTTACAGAA41360.12916765561954255No Hit
CTGCCATTGCACAGTT41270.12888658480218862No Hit
TGGAGTTACAAGTCTC40780.1273563103521505No Hit
TTGTAACCTCATCCTC40420.12623202708273473No Hit
GGTAGCCTGTGCCACG40200.12554496508475846No Hit
TGTTTGTTGCGCTCGA40190.12551373499394136No Hit
GATACCATGTCTGGAT40150.12538881463067295No Hit
GGCTTGCGAGTACGTT40060.12510774381331902No Hit
ATAGCCAACATGGCCC40060.12510774381331902No Hit
CCTACGGGATGGAACG39090.12207842500405991No Hit
ATCTTACTGCGCACTG39090.12207842500405991No Hit
CGCTTACTGGCCACGT38940.12160997364180336No Hit
CGCTGAGTGTTAGCTG38880.12142259309690072No Hit
CTGATTACTGCTTTGA38660.12073553109892443No Hit
GGGATCAGATGAGTAA38130.11908033628561791No Hit
CGATCAAACCGATCGC38120.1190491061948008No Hit
TCAGGCTCTTAAAGCA37930.11845573446927585No Hit
CATGTTACTAACGTGG37540.11723776092740879No Hit
TGGGTGCACTTCTGAG36890.115207805024297No Hit
TCCAAAGTGAAGGCCA36680.11455197311713783No Hit
CGCTTCCCTGCGCTGT36640.11442705275386941No Hit
CTCGGATCTTTCTGAG36580.11423967220896677No Hit
CCTCCCTTGTTTCGCC36120.11280308803137999No Hit
AGCCGGTACGCGCAAA35460.11074190203745113No Hit
GCATAGTACGCTCCTT35430.11064821176499981No Hit
TTCTAACACTACGTTG35280.11017976040274326No Hit
CTTAGGCCTTATGACC35140.10974253913130379No Hit
AACAGCTACTCGAGAG35040.10943023822313275No Hit
ACCTGCTTGCCAAACT34890.1089617868608762No Hit
CCTTCAGTGTAGCTTG34880.10893055677005908No Hit
TACAACCGATTCCATT34690.10833718504453412No Hit
TGAGATAGACTCATCC34470.10765012304655781No Hit
TATCTCCCTGGAATTA34430.10752520268328941No Hit
ATTGAGAGATGATCAC34390.107400282320021No Hit
AGTGGTACTCAACGCC34290.10708798141184994No Hit
GTCCTGACTTTACGAC34260.10699429113939862No Hit
ATCGCAATGTAACTCG34140.10661953004959339No Hit
AACCACTGAAAACATG34070.10640091941387367No Hit
AGAACATGAAAGCTAA33800.10555770696181184No Hit
AGAACAATGAGTCGGA33760.10543278659854342No Hit
TACGCCTTGGGTTGAG33610.10496433523628687No Hit
CATGTCAGAACAGGTG33570.10483941487301844No Hit
CGAGTCTCTTACTTTC33300.10399620242095664No Hit
TGGTCCTTGTTTCTGT33080.10330914042298035No Hit
GCATGGGCTATGTGGG33000.10305929969644352No Hit
TTCACGCTGTGGGCAA32970.1029656094239922No Hit
CTAGAGACTCCGTTAA32490.1014665650647712No Hit
GTGTACTGAATCACAC32190.10052966234025808No Hit
GTAAGAAACGACAAAT32170.10046720215862387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)