FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004947970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653938
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT355670297.33887110290323No Hit
TCACTCCA225690.6176623686554069No Hit
TCCCTCCT117210.3207771998320716No Hit
ACACTCCT112160.30695649460937763No Hit
TCACACCT78540.2149461758792842No Hit
TCACTACT70950.19417406644557186No Hit
TAACTCCT46580.12747890084615557No Hit
TCAATCCT45800.1253442176632444No Hit
TCACTCAT39860.1090877841933826RNA PCR Primer, Index 45 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)