FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004947971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14290589
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1570081.0986810970492538No Hit
TGCTGCTACCGTTCTC1449041.0139819989225076No Hit
AAACGACCTCTTCAAG1417600.9919815061506563No Hit
ATGGGTTACAATCGAA1369350.9582180272625572No Hit
TATGCTTTGGACTGTT1346970.9425573711482431No Hit
GCCTCTTGATCTGTGA1296230.907051486821152No Hit
AAAGCGGACTCGGGCT1254780.8780463842323083No Hit
TCCAAAGTGCTCACGG1196460.8372363098539886No Hit
TAGTGCATGAACCCGC1122210.7852790392334423No Hit
GCAGGTCTGGCTGTTA1087520.761004322495035No Hit
TAACTCACTGGTGTCC1068000.7473449834712901No Hit
TGTGGCTCTTACGATC1058120.7404313426129602No Hit
GAAGTATGAACAGGTG1023710.7163525590162869No Hit
TTCCCATCTCTTTGTT961720.67297436095881No Hit
GAGCCTGACCGAGTTT924260.6467613056396765No Hit
TCACCTGACTCAATGC906470.6343125535273598No Hit
GGAACTACTACCAAGG896300.6271959819150911No Hit
TGAGAATGAGCAGTTT880890.6164126615075138No Hit
ATGTCCAGAGTCGGTC847680.593173591375415No Hit
CTAGAGACTAATCTGC846390.5922708994009974No Hit
TTCGTCCGACCTGTGG823600.5763233411862869No Hit
TCGTATACTGCTAATC820730.5743150264835131No Hit
TCACACAGATTGGGAG819870.5737132318339013No Hit
ATTCCTAACTGCTACA796860.5576117261506857No Hit
TCCGCGAGAGAACAGC781320.546737436784446No Hit
AGGTCGAGAAGGGAGG773370.5411743350816401No Hit
ACTGATTCTCGGCTCA768550.5378014859989326No Hit
TGGACCTCTAGGGAGG755950.5289844946209006No Hit
TGGCCTGGACCCGAGT749070.5241701374240069No Hit
CCATGATGATTCGGGC737950.5163887926522832No Hit
GCAATCATGGAACGTG723980.5066131284021953No Hit
TTCGATGGAAATCCTG656070.4590923439194844No Hit
ACGAGGATGGAGCTGA644760.4511780445158699No Hit
AAGGTGCGAACAGATA621530.4349225913641488No Hit
TCCCAGACTGTCTTGA617420.43204657274798125No Hit
TTTCGTAACAGTACGG615820.43092695479521526No Hit
TGCGGTGACCTTAGTG607940.4254128363778428No Hit
TGGTCCTTGGAGAGGG600480.4201926176730714No Hit
TCTCTCTACTCTGGAT588810.4120264042300845No Hit
TCCTTCACTTGTCACA525640.3678224879324428No Hit
TTCGATGGACAGGTGA513390.3592504129815783No Hit
TGATATCCTCGATGTT511210.35772493352093465No Hit
AGCCCAGTGAAAGTAC510110.35695519617840804No Hit
AGCTGCTCTTGTCACA508160.35559066179847454No Hit
TCGCCGAGAAGGTAAT505460.35370130650318193No Hit
AACTCCCTGCTTCACC499340.34941876783385206No Hit
CTTTCGGTGCTTTGTT487880.3413995042471657No Hit
AACCCTCACGCGGTTC486290.34028688390660455No Hit
CCCTTGCCTCCTACGG471150.3296924990285565No Hit
ATGGCCGTGATACGCT468330.3277191723868064No Hit
GAAGTAATGAGTCTGT462520.323653559695825No Hit
TCGGCTTACCTCCTAC446810.31266031092210406No Hit
TGTTTGTTGCAAGTTC438590.30690827368976886No Hit
AAACACGCTGCAGTTT431830.3021778878393326No Hit
ACCCTTGTGGGCCATC420230.29406065768177925No Hit
CTAACTGGAACCTTGA418400.2927800946483032No Hit
TGGCCTTTGCGAAAGC417510.29215730716207705No Hit
GGGGGGGGGGGGGGGG416190.29123362235104516No Hit
TACAACGTGAGCAGGT408720.28600640603406896No Hit
CAATGTGTGGTTGACT402830.2818848124454492No Hit
AAATAGGACTCTATCT399670.2796735669887364No Hit
TCAAAGGCTCTTAGTG397990.27849796813833216No Hit
ACAGCCGACAAACATG389070.27225609805166184No Hit
GGACATTTGGCCGCTA377570.26420884401615635No Hit
CTTTCGGTGAGCCATT375760.2629422762070899No Hit
TGAACTGTGTGCTGGC365400.2556927499629302No Hit
AGTGTTCGATTCGCCT351430.2459170857128422No Hit
CAGCCGATGTAATAGG346230.2422783273663528No Hit
GGCTTGCGAATACGCT336490.2354626530788899No Hit
GATAAGAACATTGGTC312420.2186194005019667No Hit
GTAAAGCCTGAAATCA302740.21184571188773255No Hit
GTGCTAATGGGAGTCA302520.21169176441922724No Hit
TTGGGAGGAATCTGTG296400.20740922574989737No Hit
CGACGTTCTTGCTACA286800.20069151803330149No Hit
TGTACTGGAAATGCTC284300.19894211498210465No Hit
GGAACATCTTTACGAC281840.197220702379727No Hit
GGACCACTGGCTAGAG279870.19584217277538388No Hit
AGGGACCCTAATCCTG277460.1941557482340301No Hit
GGGTGAAACCGACTAT268650.18799085188161244No Hit
TCTTGAACTCGTACAT268190.1876689617201922No Hit
TAACGAGCTTACTTTC263330.18426812218866556No Hit
TACCACCTGAAACATG259250.18141309640911232No Hit
TACGTGTGAAACGCAG258650.1809932396768251No Hit
CGGATGAGAACAGGCA258520.18090227071816284No Hit
AGCTTGTGAACCGTGC251990.1763328299484367No Hit
CATGATGTGCCTAGTA248090.17360376118856963No Hit
ACTTATCTGTCAGGTT246180.17226721725745525No Hit
AACTAGCACTGCCACG240070.16799167620033018No Hit
TCGGACCGAATGTCCA229500.16059520009986994No Hit
ACGTGTTGATCTTTGT225340.1576841934226784No Hit
AACTACGACATTGCCA222420.15564089065888048No Hit
TGGTAGGGATGTGCTA218610.15297480040885647No Hit
GTAGATAGAGCTGAGA217630.15228903441278732No Hit
CGTCGTACTTGCGCCA217030.1518691776805001No Hit
AACGAACCTGACACCC215130.1505396313615905No Hit
TTTAGGGACTGGGCAA212050.148384366802516No Hit
GGTAGTTTGTCCAGCG209860.14685188972966753No Hit
CTCGGATCTAGTACGG208070.14559931714501062No Hit
CCTTCAGTGCAACGCC208030.14557132669619147No Hit
GTCCGAGTGGTCTTGA207450.1451654651883138No Hit
GCCATAATGACCGTTA206440.14445870635563027No Hit
TACCACCTGTACTTTC206390.14442371829460635No Hit
AGTTTGCGAGCAGGAT206050.14418579947964358No Hit
TGAGGAGTGGTCCCAG199820.13982628707606104No Hit
CTTTAACTGTTCGCCT195200.1365933902374493No Hit
CAGGCCATGTCGTAGT185350.12970074221573372No Hit
AGAACGCGACACAGTT185340.12969374460352895No Hit
CTTACATTGGAAGGCT183320.1282802269381619No Hit
GTCCGTTACGGATGGT181030.12667777374326558No Hit
GGGATGTGATGCATGT177200.12399768826883203No Hit
TGCAATGACTCCGTAA176990.1238507384125315No Hit
CAAAGACCTGCTTTGA176780.12370378855623096No Hit
GAAAGTCCTTATCTGG176080.12321395570189585No Hit
GAGAAGAGATGCTACA175620.12289206554047562No Hit
CGATCGGTGCGAGGAT175600.12287807031606604No Hit
GAACCATACTTTGGGT174930.1224092302983453No Hit
CCGTTACTGTTGGTTG173380.12132460040660326No Hit
AACTACGACGCTGTTA169880.11887543613492768No Hit
GCTGGACACATTGGTC165350.11570551780615901No Hit
AATTCTGCTTAGCGAG164200.11490079240260846No Hit
TCCTCATCTTTCTGGA164080.114816821056151No Hit
GGCTTAAACTGCATCA163090.11412405744787706No Hit
GAGGTTGGAGGAATAT159750.11178685497147808No Hit
ATGGCCGTGGGCCAAA158750.11108709375099934No Hit
TCGTAGAACCGCACTG158510.11091915105808446No Hit
TGTCTTCCTGAAATCA158240.11073021552855519No Hit
CCCACCTCTTGACCTA157950.11052728477461636No Hit
TCCGTAAGATTACGCA154900.10839301305215622No Hit
CCTGAGGACTGACTCA152970.10704247389663225No Hit
ACAAGACCTAAGCTAA152730.10687453120371736No Hit
ATAGCCAACATCTGTG151930.10631472222733436No Hit
TATGGGTACCCTTTGC150390.10523708994779712No Hit
AGGTGATCTGTTAGTC145760.10199719549698057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)