FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936327
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA287617997.95159054151667No Hit
GTGGCGCA138870.4729377892857301No Hit
GGGGAGCA53750.1830518195010297No Hit
GAGGAGCA38750.1319675908030679No Hit
GTGGAGAA37410.12740406637271667No Hit
GTGGAGCC34470.11739155754791615No Hit
GTGGAGCT34040.11592714299190791No Hit
GTGGACCA31310.10662981336887888ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGCAGCA30210.102883636597695No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)