FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3688879
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC358730497.24645346187826Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA222220.6024052293393196No Hit
ATCTGCTC160950.43631141059384165No Hit
ATCAGATC101390.2748531464436757Illumina PCR Primer Index 9 (100% over 8bp)
ATATGATC74750.2026360853798674No Hit
ATCTGAAC67820.18384989044097136No Hit
AACTGATC62860.17040407126392598No Hit
ATCTCATC52910.1434311073906192No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)