FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221496
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT384281.7298253069102982No Hit
GAAGCAGTGGAACCTT133580.6013065069664767No Hit
GTTCTTCCTAACGTAA117800.5302732933122545No Hit
GATACGTACCGCTCGA114460.5152383799025522No Hit
CGCTTAAGATAATAGG88990.4005859114758703No Hit
CATGGAGGATCGACAT88700.3992804848624531No Hit
TGTCCCATGAGACAAT86570.3896923514604573No Hit
AAAGCATGATCTTTGT82070.3694357315970859No Hit
TGAGCGCACAGGACAC80300.3614681277841598No Hit
TAATTGCCTGTCTGGC74810.33675505155084684No Hit
TGTCGAAACTTCGCAA74220.3340991836132048No Hit
GGAAGGACTAGGTAAT64500.2903448847083227No Hit
GGGGGGGGGGGGGGGG60000.2700882648449513No Hit
AATGATGGAAAGCTAA58480.2632460288022126No Hit
CATGTCAGAGGTTTGT57420.25847446945661845No Hit
TCGACCTTGTTTCGCC56050.25230745407599203No Hit
GCTATGATGACCTGCG45860.2064374637631578No Hit
ATGGGTACTTGGCTGC45040.20274625747694347No Hit
AAACGACCTAGGGAGG43200.19446355068836496No Hit
AGCCTACCTTAACTCG41560.1870811381159363No Hit
CAGCTCACTGTTTACG41370.18622585861059396No Hit
TGTTCCTCTTTATGGC40980.18447028488910175No Hit
TTGCGATGACATTCAG40170.18082409331369492No Hit
GTGAGATCTCGATTAC39670.1785733577733203No Hit
CTGCCTTGACCATAAC37950.1708308275144317No Hit
CCTGTTGACCCTAGTA37050.16677950354175744No Hit
CACAGCTCTGGACTGA36670.16506894453107276No Hit
TAACCAGACATCACAC36000.1620529589069708No Hit
CAATGTGACCAACGCC35920.16169284122051086No Hit
CATGATGTGTCATGTG35880.1615127823772809No Hit
AGCTTGTGACTCACTC35740.160882576425976No Hit
CATGCAAACGAAGGCT35100.15800163493429653No Hit
ATGCCGCGATGCCACG34450.15507567873180955No Hit
CTATTCAACCGAACGA34210.15399532567242977No Hit
TGGGCCATGCGTTCTC34050.1532750902995099No Hit
ATGGGTACTTTCGGGC33760.1519696636860926No Hit
ATTAGCGTGGTCGGTC33370.15021408996460042No Hit
TTGTTTGTGATACGCT32960.14836848682149326No Hit
AGAACGCGAGGAATAT32130.14463226582447145No Hit
CACTATACTACGCCAC32060.144317162848819No Hit
GGGCTGACTAATACAG31570.14211144201925188No Hit
GGGTCGTACCGCTCGA31170.1403108535869522No Hit
GCAGATACTCGGCTCA31110.14004076532210727No Hit
CCGCTATTGTTCGCAA30910.13914047110595743No Hit
CATTAGTCTATTCTGG30900.13909545639514995No Hit
GCATCTCTGAGCCTAT30510.13733988267365774No Hit
CCCAATGCTGGAATGC30070.1353592353981281No Hit
AACGTCCCTTACAGAA29390.13229823506321867No Hit
ATAGGAAACAGACTTC29210.1314879702686838No Hit
GGCTTGCGAGTACGTT29180.13135292613626134No Hit
TTGACGTGACCTCAAA29060.1308127496065714No Hit
GATACCATGTCTGGAT29060.1308127496065714No Hit
AGCTCGGACCTTAAGC28790.12959735241476916No Hit
GGTAGCCTGTGCCACG28770.12950732299315415No Hit
AACCAACACCGGAATG28420.12793180811489194No Hit
TGGAGTTACAAGTCTC28260.12721157274197206No Hit
CTGATTACTGCTTTGA28210.12698649918793461No Hit
TTGTAACCTCATCCTC28120.1265813667906672No Hit
ATAGCCAACATGGCCC27850.12536596959886492No Hit
CTGCCATTGCACAGTT27810.12518591075563493No Hit
CCTACGGGATGGAACG27760.12496083720159748No Hit
GGGATCAGATGAGTAA27680.12460071951513754No Hit
TCAGGCTCTTAAAGCA26710.12023429256681083No Hit
ATCTTACTGCGCACTG26540.11946904248308347No Hit
TGTTTGTTGCGCTCGA26490.11924396892904603No Hit
CGATCAAACCGATCGC26410.11888385124258609No Hit
CGCTTACTGGCCACGT26410.11888385124258609No Hit
TCCAAAGTGAAGGCCA26200.11793854231562874No Hit
CGCTGAGTGTTAGCTG26050.11726332165351637No Hit
CATGTTACTAACGTGG25850.11636302743736654No Hit
CTCGGATCTTTCTGAG25580.11514763024556426No Hit
TTCTAACACTACGTTG25480.11469748313748933No Hit
TGGGTGCACTTCTGAG25470.11465246842668185No Hit
CGCTTCCCTGCGCTGT25170.11330202710245708No Hit
AGCCGGTACGCGCAAA25110.11303193883761213No Hit
CCTCCCTTGTTTCGCC24650.11096126214046749No Hit
GCATAGTACGCTCCTT24520.1103760708999701No Hit
CCTTCAGTGTAGCTTG24130.10862049717847792No Hit
ACCTGCTTGCCAAACT24100.10848545304605545No Hit
CTTAGGCCTTATGACC23940.10776521767313559No Hit
GTCCTGACTTTACGAC23920.1076751882515206No Hit
TACGCCTTGGGTTGAG23910.10763017354071311No Hit
TGAGATAGACTCATCC23860.10740509998667565No Hit
AACCACTGAAAACATG23830.10727005585425317No Hit
GCATGGGCTATGTGGG23820.10722504114344569No Hit
TACAACCGATTCCATT23750.10690993816779323No Hit
TTCACGCTGTGGGCAA23620.10632474692729584No Hit
GTAAGAAACGACAAAT23550.1060096439516434No Hit
AGTGGTACTCAACGCC23470.10564952626518347No Hit
TATCTCCCTGGAATTA23470.10564952626518347No Hit
GTGTACTGAATCACAC23390.10528940857872354No Hit
ATTGAGAGATGATCAC23390.10528940857872354No Hit
AGAACAATGAGTCGGA23310.1049292908922636No Hit
AACAGCTACTCGAGAG23270.10474923204903362No Hit
ACCTCCATGGAATTGA23100.10398398196530625No Hit
CATGTCAGAACAGGTG23080.10389395254369127No Hit
GAAAGTAACCCTGCGA23040.10371389370046132No Hit
TTCTATGACCAACGCC22750.10240846708704406No Hit
TGGTCCTTGTTTCTGT22690.1021383788221991No Hit
ATCGCAATGTAACTCG22680.1020933641113916No Hit
CGAGTCTCTTACTTTC22570.10159820229250918No Hit
GCATATCGAGCCGCTA22460.10110304047362677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)