FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714653
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC211990.5706858756389898No Hit
CTCCTAGTGAACGTAA193590.5211523122078967No Hit
CGACCAGACGCGCTGT187440.504596257039352No Hit
TAGCTTTACCCTATCC169340.45587030605550505No Hit
CCAGGACTGTAACTCG130980.3526035944676394No Hit
GTTCTCGACCGTAGAG120730.3250101691867316No Hit
ATCTGTACTGGGACTA120710.3249563283569152No Hit
CTTCCTTTGGCTCAGC118150.31806470214041527No Hit
CGATCAAACCCGGCTA107620.2897175052420778No Hit
NNNNNNNNNNNNNNNN106240.2860024879847458No Hit
AGACCATTGAATCCAC105340.28357965064300755No Hit
TGCTGCTACTTTCTTG95860.25805909731003135No Hit
GGCAACAGAATAGCAA92890.2500637340822952No Hit
ACAGCCGACTTGCGTA90900.24470657151556283No Hit
GGGGGGGGGGGGGGGG90560.24379127740868392No Hit
AACTTACCTCACCTAA82320.22160885552432485No Hit
CGCGTTGGAGCGGTTC78070.21016767918833873No Hit
ATCGAAAGATTTCCGC75840.20416442666380952No Hit
GTAACTGTGTGAAAGT72970.19643826758515534No Hit
CCCTGAACTAGCTTTG70220.18903515348539957No Hit
AGTGGAGTGAGTTGAA69880.1881198593785207No Hit
CAATTCGGATAACGGG67940.18289729888632936No Hit
GGGTAGAACCAGTCTA64650.17404048238153066No Hit
AAGAACCCTTAACCCA62300.16771418487810302No Hit
TTCCTAAACTAATGCC61400.16529134753636476No Hit
GTGAAGCGATCCCAGG61010.16424145135494486No Hit
CGCGATCGATATCTGG58660.15791515385151722No Hit
TGGAACACTCCTAGTA58350.15708062098936293No Hit
TTTAGTCTGAGCAATA57070.15363480788111297No Hit
TGATAGAGAGACTGTT55930.15056588058157788No Hit
TGCAGATACTGCCACG55440.14924678025107593No Hit
TCCGGGAACGTTCTCC55320.1489237352721775No Hit
CTTTAACTGTCCGTAA54890.1477661574311248No Hit
AGTCCTGCTCGAACGA54780.14747003286713456No Hit
CACATCCTGAGCCTAT54420.14650089793043927No Hit
GCAACTTGAAGGTGCA52680.14181674573641198No Hit
TGCAGTATGACACCGC52250.1406591678953593No Hit
AGGTGTGGATCATGTG51810.13947466963939836No Hit
CATAGGTACAAGGTGC51480.13858629594742766No Hit
TCTCAGAGATGCATGT51130.13764408142564055No Hit
CTTAGATCTTGTCACA50270.13532892574353514No Hit
TACGTTTACTGCACCG49780.1340098254130332No Hit
GAGTGTTACACCTGCG49340.13282532715707226No Hit
TCAAAGCACTCTGCAA49090.1321523167843672No Hit
ATTTCTGACGGGTAGT48690.1310755001880391No Hit
CTATTGGGACTTGTGA48320.1300794448364356No Hit
AGAGTACCTTAATCGC48180.12970255902772077No Hit
ATGTACGTGTACTTTC48130.12956795695317974No Hit
TGGCAATTGAGTGGTG47170.12698359712199228No Hit
GGTCTAAACTCCTACC46920.12631058674928722No Hit
TGAACTGTGAACGTAA46850.1261221438449298No Hit
TCTATGGGATGCTCAA46700.12571833762130674No Hit
TTCTTAGACGTCCCGA46500.1251799293231427No Hit
CGAGTCTCTGCTACGC46370.12482996392933607No Hit
GGTCGCATGGTACTAC45900.12356470442865053No Hit
GGGTGTGCTCGCAAAG44870.12079190169310566No Hit
GCATGATACTTGCGTA44490.11976892592659395No Hit
CTACAACGAATCTGTG44310.11928435845824631No Hit
TACTGTTTGCTTAAGC44280.11920359721352168No Hit
TCCATTTGAAATCTGC43440.11694228236123266No Hit
CACCAGGTGCGCCACA43020.11581162493508816No Hit
CATTCTATGCCTAGCG42330.11395411630642216No Hit
TGAGGGATGTACGTTG42190.11357723049770732No Hit
GGGATGTGACTGGAAG42180.11355031008279913No Hit
GTAATCTCTACACAAA41980.11301190178463506No Hit
TTGCGGTCTTTTGGGT41940.11290422012500226No Hit
CTAGCTCACGAATAGT41690.11223120975229718No Hit
TCGGACCGATAGGCGA41640.11209660767775617No Hit
CTCAATTTGGAATCTC41640.11209660767775617No Hit
AGACTACTGGCCCTAG41290.11115439315596908No Hit
CGAGTCTCTTGGAACG41010.1104006215385394No Hit
AGCCAATTGTTATGGC40340.10859695373968982No Hit
GGCTGACTGAAACTGA40260.1083815904204242No Hit
TGTAAGCACGCGCTGT39980.1076278188029945No Hit
TCCGGGTGAACACCTA39900.10741245548372891No Hit
CTAACAGTGCATGATC39830.10722401257937147No Hit
ACCAAGTTGAAGCTAA39690.10684712677065664No Hit
TGCGCAGACATCAGGG39670.10679328594084023No Hit
ATGTCCAGACCGCTAG39660.10676636552593202No Hit
CACATTACTGATGGAC39650.10673944511102383No Hit
CATAGTCGAAGATTCC39270.10571646934451212No Hit
TGCTAGTGAAGGCGTA39240.10563570809978752No Hit
ACCTCAGCTAATCCGT39110.10528574270598089No Hit
CTTTCGTCTCGAACGA39090.10523190187616448No Hit
AGGCGATGACCTGCGA38930.10480117523763324No Hit
GGGCCTTGACTATCTT38690.10415508527983636No Hit
TAGTACCGAACGAGCA38690.10415508527983636No Hit
GCCATTCACCGGAAGT37540.10105923756539305No Hit
AAAGCCTTGAACCAAA37480.10089771507594385No Hit
GTCTAATCTAAGTTCC37390.10065543134177002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)