FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30784714
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450030.1461861883790767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG36500.08.5898685
TACACTG54000.08.320375
TAGACTG42850.08.2642045
TAGACAG54900.07.92901755
CCTACAC50200.07.8881043
GTATTAG32550.07.60718251
GTGTATA57950.07.58014971
TACACAG69250.07.579545
CTAGGAC45350.07.4975423
GTATAGA28850.07.46752071
GTGTAGG49550.07.45352651
GTCTAGG43600.07.44394351
CTAGTAC19550.07.37191633
CCTATAC24700.07.36438233
GTCCTAG44650.07.3628841
TAGGACT43450.07.24671754
GTCTTAG38350.07.22272971
GTATAGG26250.07.1412961
CTACACT55700.07.0850434
GTGTTAG51200.07.07670971
Produced by FastQC (version 0.11.5)