FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139412
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT497978396.89402211770529No Hit
CTAGCTGA304920.5932974433651165No Hit
CTCGCTGT108520.21115255986482498No Hit
CTAGCAGT105910.2060741578997753No Hit
CAAGCTGT95900.18659722162768813No Hit
CTAGATGT95460.18574109256078322No Hit
CTAGCTGG91870.17875585767399071No Hit
CTAGCTAT87450.17015565204735483RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCTCT83370.16221700069969094No Hit
ATAGCTGT78510.15276066600615013No Hit
CTAGCGGT76600.149044287556631No Hit
CTACCTGT76240.1483438183200724No Hit
CTTGCTGT69040.13433443358890082No Hit
CTAGCTTT59110.11501315714716003No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)