FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4121903
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCTACCGTTCTC409210.9927696018077087No Hit
GCACTGTCTCTTTGTT405870.9846665484364867No Hit
AAACGACCTCTTCAAG405510.9837931654383909No Hit
ATGGGTTACAATCGAA383680.9308321908594162No Hit
TATGCTTTGGACTGTT383130.9294978557234366No Hit
AAAGCGGACTCGGGCT356870.8657894181401165No Hit
GCCTCTTGATCTGTGA343830.8341535450979802No Hit
TCCAAAGTGCTCACGG334430.811348544592146No Hit
GCAGGTCTGGCTGTTA333870.8099899488173303No Hit
TAGTGCATGAACCCGC329800.8001158688110808No Hit
TAACTCACTGGTGTCC308260.7478584527583497No Hit
TGTGGCTCTTACGATC300100.7280617714681786No Hit
GAAGTATGAACAGGTG285360.6923015898239235No Hit
TTCCCATCTCTTTGTT269890.654770381544641No Hit
TCACCTGACTCAATGC257330.6242990191666324No Hit
GGAACTACTACCAAGG252420.6123870454981595No Hit
ATGTCCAGAGTCGGTC248760.6035076516841857No Hit
TGAGAATGAGCAGTTT244820.5939489599828041No Hit
TCGTATACTGCTAATC240470.5833955820891467No Hit
TTCGTCCGACCTGTGG237910.5771848585471322No Hit
CTAGAGACTAATCTGC236440.5736185446382411No Hit
GAGCCTGACCGAGTTT235530.5714108265041656No Hit
TGCGGTGACCTTAGTG230920.5602266720007725No Hit
TCACACAGATTGGGAG229070.5557384538161135No Hit
GAAGTAATGAGTCTGT226450.5493821664410832No Hit
ATTCCTAACTGCTACA225000.5458643738098641No Hit
TCCGCGAGAGAACAGC222360.5394595651571616No Hit
ACTGATTCTCGGCTCA221790.53807670874351No Hit
AGGTCGAGAAGGGAGG219380.5322298947840355No Hit
TGGCCTGGACCCGAGT215460.5227197243603258No Hit
TGGACCTCTAGGGAGG211000.5118994794394726No Hit
CCATGATGATTCGGGC202260.4906956810968138No Hit
ACGAGGATGGAGCTGA187280.45435324412049477No Hit
GCAATCATGGAACGTG185920.4510537972387997No Hit
TTCGATGGAAATCCTG183740.445764977972553No Hit
AAGGTGCGAACAGATA177940.43169380744767644No Hit
TCCCAGACTGTCTTGA176520.42824879673296534No Hit
TTTCGTAACAGTACGG174690.4238090998259785No Hit
TGGTCCTTGGAGAGGG170820.4144202325964488No Hit
TCTCTCTACTCTGGAT161910.3928040033935782No Hit
TCCTTCACTTGTCACA152610.3702416092761038No Hit
TCGCCGAGAAGGTAAT147000.35663139088911117No Hit
TTCGATGGACAGGTGA146320.3549816674482636No Hit
TGATATCCTCGATGTT144420.3503721460694247No Hit
AGCCCAGTGAAAGTAC143890.34908633221111707No Hit
GCGGTTCGAGTACGTT143680.3485768587955612No Hit
AGCTGCTCTTGTCACA140950.34195370439333483No Hit
AACCCTCACGCGGTTC139110.33748974684751193No Hit
AACTCCCTGCTTCACC137470.33351100207840895No Hit
CTTTCGGTGCTTTGTT137020.3324192733307892No Hit
CCCTTGCCTCCTACGG134380.3260144646780868No Hit
ATGGCCGTGATACGCT132620.3217445922429518No Hit
TCGGCTTACCTCCTAC127470.309250363242415No Hit
TGTTTGTTGCAAGTTC125620.30476214505775606No Hit
AAACACGCTGCAGTTT121710.2952762352728825No Hit
CTAACTGGAACCTTGA118490.28746430956769237No Hit
ACCCTTGTGGGCCATC118430.2873187457346764No Hit
TGGCCTTTGCGAAAGC117770.2857175435715008No Hit
TACAACGTGAGCAGGT116540.2827334849946736No Hit
AAATAGGACTCTATCT115540.28030742111107415No Hit
TCAAAGGCTCTTAGTG114640.27812396361583475No Hit
CAATGTGTGGTTGACT112310.2724712347670481No Hit
GGGGGGGGGGGGGGGG111250.26989960705043275No Hit
ACAGCCGACAAACATG107970.26194211751222674No Hit
CTTTCGGTGAGCCATT105300.2554645269430164No Hit
TGAACTGTGTGCTGGC103280.2505638778981456No Hit
CAGCCGATGTAATAGG100120.2428975160259715No Hit
AGTGTTCGATTCGCCT99040.24027736703168415No Hit
GGACATTTGGCCGCTA95020.2305245902196146No Hit
GAGGTTGGAGGAATAT87290.21177111639939125No Hit
TTGGGAGGAATCTGTG84260.2044201428320851No Hit
TGTACTGGAAATGCTC83590.2027946800300735No Hit
GTGCTAATGGGAGTCA83060.2015088661717658No Hit
CGACGTTCTTGCTACA82820.20092661083970198No Hit
TCTTGAACTCGTACAT81080.196705259682239No Hit
GGAACATCTTTACGAC80340.19490997240837546No Hit
AGGGACCCTAATCCTG77160.18719508925852937No Hit
TAACGAGCTTACTTTC75930.1842110306817021No Hit
CGGATGAGAACAGGCA75400.18292521682339444No Hit
TACCACCTGAAACATG75070.18212461574180663No Hit
GGACCACTGGCTAGAG74970.1818820093534467No Hit
GGGTGAAACCGACTAT72210.17518607303471234No Hit
TACGTGTGAAACGCAG71400.17322096128899686No Hit
CATGATGTGCCTAGTA69830.16941204099174578No Hit
AGCTTGTGAACCGTGC69410.16839309416063405No Hit
TCGGACCGAATGTCCA68550.16630667922073858No Hit
ACTTATCTGTCAGGTT67100.16278888658951945No Hit
ACGTGTTGATCTTTGT66280.16079951420496794No Hit
TGGTAGGGATGTGCTA65850.1597563067350202No Hit
AACTAGCACTGCCACG65840.15973204609618422No Hit
CTCGGATCTAGTACGG63800.15478287577364144No Hit
GTCCGAGTGGTCTTGA62560.1517745565579782No Hit
GTAGATAGAGCTGAGA62190.15087691292104644No Hit
CGTCGTACTTGCGCCA61920.15022187567247458No Hit
AACGAACCTGACACCC61360.14886327989765893No Hit
TTTAGGGACTGGGCAA61210.148499370315119No Hit
AACTACGACATTGCCA61160.14837806712093904No Hit
GGTAGTTTGTCCAGCG59480.14430227979649207No Hit
CCTTCAGTGCAACGCC59030.14321055104887231No Hit
TGAGGAGTGGTCCCAG58570.1420945616624166No Hit
TACCACCTGTACTTTC58170.14112413610897684No Hit
AGTTTGCGAGCAGGAT57980.14066318397109298No Hit
CTTTAACTGTTCGCCT56100.13610218386992612No Hit
GCCATAATGACCGTTA54620.132511609322199No Hit
CGATCGGTGCGAGGAT53840.13061927949299146No Hit
CAGGCCATGTCGTAGT52980.12853286455309598No Hit
CTTACATTGGAAGGCT52410.12715000813944433No Hit
AGAACGCGACACAGTT51680.1253789815044168No Hit
CAAAGACCTGCTTTGA51460.12484524745002491No Hit
GGGATGTGATGCATGT51410.12472394425584493No Hit
GTCCGTTACGGATGGT51190.12419021020145307No Hit
TGCAATGACTCCGTAA50560.12266178995478545No Hit
GGCTTGCGAATACGCT50160.12169136440134569No Hit
CCGTTACTGTTGGTTG49520.12013868351584207No Hit
TCCTCATCTTTCTGGA48820.1184404387973225No Hit
GAACCATACTTTGGGT48580.11785818346525864No Hit
GAGAAGAGATGCTACA48120.11674219407880292No Hit
AATTCTGCTTAGCGAG47700.11572324724769117No Hit
GAAAGTCCTTATCTGG47500.11523803447097129No Hit
TGTCTTCCTGAAATCA47230.11458299722239945No Hit
CCCACCTCTTGACCTA46900.11378239614081166No Hit
AACTACGACGCTGTTA46820.11358831103012369No Hit
TCCGTAAGATTACGCA45550.11050720989795249No Hit
TCGTAGAACCGCACTG45290.10987643328821664No Hit
ATGGCCGTGGGCCAAA45220.10970660881636468No Hit
CCTGAGGACTGACTCA43880.10645568321234147No Hit
GAAGGACACCCAGCTG42740.10368997038503819No Hit
TATGGGTACCCTTTGC42520.1031562363306463No Hit
ACAAGACCTAAGCTAA41850.1015307735286347No Hit
AGGTGATCTGTTAGTC41480.10063312989170295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)