FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306246
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG322891397.66100284128888No Hit
TAATGGTG77680.23494924455107088No Hit
TATTGGTA75100.22714583246376704No Hit
TATTGGGG65470.19801914316115615No Hit
AATTGGTG61510.1860418129806433No Hit
TATTGGTC50550.152892434501244No Hit
TATTGCTG48650.14714573567726055No Hit
TATTGGAG47100.14245763926822144No Hit
TATTCGTG45710.13825347539172828No Hit
TATAGGTG43370.1311759621032434RNA PCR Primer, Index 39 (100% over 8bp)
TTTTGGTG43250.1308130127038339RNA PCR Primer, Index 28 (100% over 8bp)
TATTGGTT42860.12963342715575307No Hit
TAGTGGTG37480.11336119574889467No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)