FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230097
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT328331.0164710223872533No Hit
TGCTGCTACCGTTCTC328331.0164710223872533No Hit
AAACGACCTCTTCAAG320180.9912395819692101No Hit
ATGGGTTACAATCGAA310540.961395276983942No Hit
TATGCTTTGGACTGTT304560.942881901069844No Hit
AAAGCGGACTCGGGCT285630.8842768498902664No Hit
GCCTCTTGATCTGTGA277490.8590763682948221No Hit
GCAGGTCTGGCTGTTA270470.8373432748304462No Hit
TCCAAAGTGCTCACGG268250.8304704162135069No Hit
TAGTGCATGAACCCGC254900.7891403880440743No Hit
TAACTCACTGGTGTCC243340.7533519891198314No Hit
TGTGGCTCTTACGATC240460.7444358482113695No Hit
GAAGTATGAACAGGTG236970.7336312191243792No Hit
TTCCCATCTCTTTGTT217090.6720850797979132No Hit
TCACCTGACTCAATGC208450.6453366570725275No Hit
TGAGAATGAGCAGTTT197340.6109414051652319No Hit
GGAACTACTACCAAGG195540.6053688170974433No Hit
GAGCCTGACCGAGTTT192570.5961740467855919No Hit
ATGTCCAGAGTCGGTC191210.5919636469121515No Hit
TCGTATACTGCTAATC188510.5836047648104686No Hit
TTCGTCCGACCTGTGG187250.5797039531630165No Hit
CTAGAGACTAATCTGC186080.5760817709189539No Hit
TCACACAGATTGGGAG181730.5626146830884645No Hit
TGCGGTGACCTTAGTG180970.5602618125709538No Hit
GAAGTAATGAGTCTGT180870.5599522243449655No Hit
ATTCCTAACTGCTACA178520.5526769010342414No Hit
TCCGCGAGAGAACAGC177170.5484974599833998No Hit
ACTGATTCTCGGCTCA174270.5395194014297403No Hit
AGGTCGAGAAGGGAGG173400.5368259838636424No Hit
TGGACCTCTAGGGAGG170260.5271049135676111No Hit
TGGCCTGGACCCGAGT169590.5250306724534898No Hit
CCATGATGATTCGGGC167200.5176315138523704No Hit
GCAATCATGGAACGTG154670.4788401091360414No Hit
ACGAGGATGGAGCTGA147760.45744756272025267No Hit
TTCGATGGAAATCCTG145630.45085333350670276No Hit
AAGGTGCGAACAGATA141670.43859363975756765No Hit
TTTCGTAACAGTACGG139990.4333925575609649No Hit
TCCCAGACTGTCTTGA138330.4282533930095598No Hit
TGGTCCTTGGAGAGGG135860.42060656382764977No Hit
TCTCTCTACTCTGGAT135460.41936821092369675No Hit
TCGCCGAGAAGGTAAT118870.3680075242322444No Hit
TTCGATGGACAGGTGA117660.36426150669778645No Hit
TCCTTCACTTGTCACA116770.36150617148649095No Hit
TGATATCCTCGATGTT115380.35720289514525416No Hit
AGCCCAGTGAAAGTAC115130.3564289245802835No Hit
GCGGTTCGAGTACGTT114560.3546642716921504No Hit
AGCTGCTCTTGTCACA114470.354385642288761No Hit
AACTCCCTGCTTCACC113500.35138263649667484No Hit
CTTTCGGTGCTTTGTT113250.3506086659317042No Hit
AACCCTCACGCGGTTC106600.33002104890348494No Hit
ATGGCCGTGATACGCT106120.3285350254187413No Hit
CCCTTGCCTCCTACGG105300.32599640196563756No Hit
TCGGCTTACCTCCTAC102890.31853532571932053No Hit
TGTTTGTTGCAAGTTC100930.3124673964899506No Hit
AAACACGCTGCAGTTT97290.30119838506397795No Hit
GGGGGGGGGGGGGGGG95990.29717373812613057No Hit
ACCCTTGTGGGCCATC95810.2966164793193517No Hit
TGGCCTTTGCGAAAGC93560.2896507442346159No Hit
TACAACGTGAGCAGGT92250.2855951384741697No Hit
AAATAGGACTCTATCT91890.28448062086061193No Hit
CTAACTGGAACCTTGA91490.2832422679566589No Hit
CAATGTGTGGTTGACT90620.28054885039056104No Hit
TCAAAGGCTCTTAGTG87740.27163270948209917No Hit
CTTTCGGTGAGCCATT85310.26410971559058444No Hit
TGAACTGTGTGCTGGC84440.26141629802448657No Hit
ACAGCCGACAAACATG84300.26098287450810304No Hit
AGTGTTCGATTCGCCT79430.24590592790247479No Hit
GGACATTTGGCCGCTA76920.23813526343016944No Hit
CAGCCGATGTAATAGG76070.23550376350926924No Hit
GAGGTTGGAGGAATAT72840.22550406380984844No Hit
CGACGTTCTTGCTACA67410.2086934231386859No Hit
TTGGGAGGAATCTGTG66950.20726931729913994No Hit
GTGCTAATGGGAGTCA66230.20504028207202446No Hit
TGTACTGGAAATGCTC64870.20082988219858414No Hit
GGAACATCTTTACGAC63990.19810550580988745No Hit
AGGGACCCTAATCCTG61140.18928224136922203No Hit
GGGTGAAACCGACTAT60950.18869402373984434No Hit
TCTTGAACTCGTACAT60950.18869402373984434No Hit
GGACCACTGGCTAGAG60480.18723895907769952No Hit
CGGATGAGAACAGGCA60170.18627923557713594No Hit
TACGTGTGAAACGCAG58480.18104719455793433No Hit
TAACGAGCTTACTTTC58370.18070664750934723No Hit
TACCACCTGAAACATG58060.17974692400878364No Hit
AGCTTGTGAACCGTGC57100.17677487703929634No Hit
ACTTATCTGTCAGGTT55400.17151187719749592No Hit
CATGATGTGCCTAGTA54900.16996393606755464No Hit
ACGTGTTGATCTTTGT52210.16163601278847045No Hit
AACTAGCACTGCCACG50940.15770424231841954No Hit
TCGGACCGAATGTCCA50780.15720890115683833No Hit
TGGTAGGGATGTGCTA50010.15482507181672872No Hit
CGTCGTACTTGCGCCA49010.1517291895568461No Hit
AACTACGACATTGCCA48640.15058371312068958No Hit
GTAGATAGAGCTGAGA48170.14912864845854473No Hit
CTCGGATCTAGTACGG48080.1488500190551553No Hit
AACGAACCTGACACCC47970.14850947200656822No Hit
GTCCGAGTGGTCTTGA47960.1484785131839694No Hit
CCTTCAGTGCAACGCC47050.14566126032747623No Hit
AGTTTGCGAGCAGGAT46390.14361797803595372No Hit
GGCTTGCGAATACGCT46250.14318455451957016No Hit
GGTAGTTTGTCCAGCG45930.14219387219640772No Hit
TTTAGGGACTGGGCAA45830.14188428397041947No Hit
TACCACCTGTACTTTC45460.1407388075342629No Hit
TGAGGAGTGGTCCCAG45170.13984100167889693No Hit
GCCATAATGACCGTTA43620.13504238417607892No Hit
CTTTAACTGTTCGCCT42930.13290622541675992No Hit
AGAACGCGACACAGTT42910.13284430777156228No Hit
CGATCGGTGCGAGGAT41050.12708596676818062No Hit
GGGATGTGATGCATGT41010.12696213147778534No Hit
CTTACATTGGAAGGCT40770.12621911973541353No Hit
GTCCGTTACGGATGGT40200.12445446684728044No Hit
CAAAGACCTGCTTTGA40120.12420679626648982No Hit
CCGTTACTGTTGGTTG39220.1214205022325955No Hit
CAGGCCATGTCGTAGT38550.11934626111847414No Hit
GATAAGAACATTGGTC38310.11860324937610232No Hit
AACTACGACGCTGTTA38250.11841749644050936No Hit
AATTCTGCTTAGCGAG38180.1182007846823176No Hit
TGCAATGACTCCGTAA37900.11733393764955047No Hit
GAACCATACTTTGGGT37430.11587887298740564No Hit
GAAAGTCCTTATCTGG37330.11556928476141738No Hit
CCCACCTCTTGACCTA37240.11529065535802795No Hit
GAGAAGAGATGCTACA36700.11361887893769135No Hit
TCCTCATCTTTCTGGA36660.11349504364729604No Hit
TGTCTTCCTGAAATCA36420.11275203190492422No Hit
CCTGAGGACTGACTCA35720.1105849143230064No Hit
ATGGCCGTGGGCCAAA35080.10860354967668154No Hit
TCGTAGAACCGCACTG34860.10792245557950737No Hit
TCCGTAAGATTACGCA34180.10581725564278718No Hit
TATGGGTACCCTTTGC34050.10541479094900245No Hit
GAAGGACACCCAGCTG33780.10457890273883416No Hit
AGGTGATCTGTTAGTC33300.10309287925409051No Hit
ACAAGACCTAAGCTAA33260.1029690439636952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)