FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517006
Sequences flagged as poor quality0
Sequence length8
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA142788194.12494083741264No Hit
TTAGTCAA678574.473087120288252No Hit
TCAGACAA34410.22682837114685112No Hit
TCAGTAAA29440.1940664704028857No Hit
TCAGGCAA29300.1931435999593937No Hit
ACAGTCAA27400.18061892965485965TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTCAT15800.10415252147980958No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)