FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3757004
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT392511.044742033812048No Hit
TGCTGCTACCGTTCTC384931.0245663832138587No Hit
AAACGACCTCTTCAAG364380.9698685441910628No Hit
ATGGGTTACAATCGAA353070.9397647700135533No Hit
TATGCTTTGGACTGTT351610.9358786948323717No Hit
AAAGCGGACTCGGGCT326820.8698952676121718No Hit
GCAGGTCTGGCTGTTA324390.863427347961301No Hit
TCCAAAGTGCTCACGG307870.8194561411166984No Hit
TAGTGCATGAACCCGC294100.7828045964284307No Hit
GAAGTATGAACAGGTG291550.7760172733380108No Hit
TAACTCACTGGTGTCC278390.7409893628007849No Hit
TGTGGCTCTTACGATC277850.7395520473228137No Hit
TTCCCATCTCTTTGTT244870.6517693353533827No Hit
GCCTCTTGATCTGTGA243900.6491874908836935No Hit
GGAACTACTACCAAGG238260.6341755292248823No Hit
TCACCTGACTCAATGC236970.6307419422497288No Hit
TGAGAATGAGCAGTTT227220.6047904127863585No Hit
ATGTCCAGAGTCGGTC226820.603725734654528No Hit
GAAGTAATGAGTCTGT226270.6022618022232609No Hit
CTAGAGACTAATCTGC223310.5943831840477146No Hit
TCGTATACTGCTAATC217740.5795575410619739No Hit
TTCGTCCGACCTGTGG215500.5735953435237225No Hit
TGCGGTGACCTTAGTG214520.5709868821007378No Hit
TCACACAGATTGGGAG207530.5523816317469984No Hit
ATTCCTAACTGCTACA205050.5457806273296488No Hit
ACTGATTCTCGGCTCA205020.5457007764697616No Hit
CCATGATGATTCGGGC204210.5435448032528046No Hit
AGGTCGAGAAGGGAGG201130.5353467816377091No Hit
TCCGCGAGAGAACAGC201010.53502737819816No Hit
GCAATCATGGAACGTG192270.511764161017662No Hit
TGGACCTCTAGGGAGG191380.5093952521743389No Hit
TGGCCTGGACCCGAGT189340.503965393702003No Hit
ACGAGGATGGAGCTGA172850.4600740377172875No Hit
TTCGATGGAAATCCTG168180.4476439205281655No Hit
AAGGTGCGAACAGATA163580.43540012201211387No Hit
TTTCGTAACAGTACGG160070.4260575714053006No Hit
TCCCAGACTGTCTTGA158570.42206502841093596No Hit
GCGGTTCGAGTACGTT154500.4112319284195598No Hit
TGGTCCTTGGAGAGGG153410.4083306805103215No Hit
TCTCTCTACTCTGGAT153160.40766525667792736No Hit
TCCTTCACTTGTCACA137580.3661960434431265No Hit
TCGCCGAGAAGGTAAT136660.36374728373991616No Hit
TTCGATGGACAGGTGA133970.3565873233033555No Hit
TGATATCCTCGATGTT133480.3552830925918631No Hit
AGCTGCTCTTGTCACA133070.35419179750673674No Hit
CTTTCGGTGCTTTGTT130080.34623332847130317No Hit
AACTCCCTGCTTCACC126920.33782237122984166No Hit
AGCCCAGTGAAAGTAC124750.3320464923646608No Hit
CCCTTGCCTCCTACGG119360.3176999545382438No Hit
ATGGCCGTGATACGCT117580.31296213685159774No Hit
TCGGCTTACCTCCTAC117240.31205716043954174No Hit
AACCCTCACGCGGTTC116590.31032705847531705No Hit
GGGGGGGGGGGGGGGG115520.30747904447267027No Hit
TGTTTGTTGCAAGTTC113760.30279446069261573No Hit
CTAACTGGAACCTTGA111150.2958474358824212No Hit
AAACACGCTGCAGTTT110050.29291957101988714No Hit
TACAACGTGAGCAGGT106120.2824591083746517No Hit
AAATAGGACTCTATCT106080.28235264056146864No Hit
TGGCCTTTGCGAAAGC105510.2808354742236101No Hit
CAATGTGTGGTTGACT104150.27721556857538615No Hit
ACAGCCGACAAACATG102180.2719720287761206No Hit
CTTTCGGTGAGCCATT100660.26792625187516433No Hit
ACCCTTGTGGGCCATC99700.265371024358771No Hit
GGACATTTGGCCGCTA99540.2649451531060388No Hit
TCAAAGGCTCTTAGTG99360.264466047946715No Hit
TGAACTGTGTGCTGGC96210.2560817076585492No Hit
CAGCCGATGTAATAGG92000.24487597032103242No Hit
AGTGTTCGATTCGCCT91850.24447671602159596No Hit
GAGGTTGGAGGAATAT91550.243678207422723No Hit
GATAAGAACATTGGTC86650.23063590030779846No Hit
GGCTTGCGAATACGCT83890.22328962119816748No Hit
GTGCTAATGGGAGTCA83320.22177245486030892No Hit
GTAAAGCCTGAAATCA80330.21381398582487535No Hit
TTGGGAGGAATCTGTG78640.20931572071789117No Hit
CGACGTTCTTGCTACA78400.20867691383879283No Hit
TGTACTGGAAATGCTC77770.20700004578115966No Hit
GGAACATCTTTACGAC75090.19986670229789483No Hit
TCTTGAACTCGTACAT70870.18863434800708226No Hit
GGGTGAAACCGACTAT70470.18756966987525167No Hit
AGGGACCCTAATCCTG69520.18504105931215403No Hit
TAACGAGCTTACTTTC69200.1841893168066896No Hit
CGGATGAGAACAGGCA69180.18413608290009806No Hit
TACGTGTGAAACGCAG67270.17905224482060708No Hit
TACCACCTGAAACATG67070.17851990575469176No Hit
CATGATGTGCCTAGTA66560.17716244113660778No Hit
AGCTTGTGAACCGTGC66470.1769228885569459No Hit
AACTAGCACTGCCACG64980.172956962515877No Hit
ACTTATCTGTCAGGTT64400.17141317922472268No Hit
ACGTGTTGATCTTTGT61830.1645726222277112No Hit
TCGGACCGAATGTCCA58900.15677385491205226No Hit
AACTACGACATTGCCA58430.15552285810715136No Hit
GTAGATAGAGCTGAGA58260.15507036990112336No Hit
TGGTAGGGATGTGCTA58230.15499051904123604No Hit
CTCGGATCTAGTACGG57500.15304748145064526No Hit
AACGAACCTGACACCC56630.15073180651391374No Hit
CGTCGTACTTGCGCCA56430.15019946744799847No Hit
GGTAGTTTGTCCAGCG56260.14974697924197047No Hit
AGTTTGCGAGCAGGAT54920.14618030750033803No Hit
TTTAGGGACTGGGCAA54450.1449293106954371No Hit
CCTTCAGTGCAACGCC53580.14261363575870561No Hit
CTTTAACTGTTCGCCT52910.1408302998878894No Hit
TACCACCTGTACTTTC52730.14035119472856564No Hit
TGAGGAGTGGTCCCAG52680.1402181099620868No Hit
CGATCGGTGCGAGGAT49690.13225964092665327No Hit
AGAACGCGACACAGTT47950.12762829105319026No Hit
GAGAAGAGATGCTACA47600.1266966976878385No Hit
GCCATAATGACCGTTA47390.12613774166862743No Hit
GGGATGTGATGCATGT47250.12576510432248675No Hit
GAAAGTCCTTATCTGG47060.12525938220986724No Hit
CAGGCCATGTCGTAGT46930.12491336181702228No Hit
CAAAGACCTGCTTTGA46620.1240882362648536No Hit
GCTGGACACATTGGTC46500.12376883282530442No Hit
GAAGGACACCCAGCTG45980.12238475125392466No Hit
CTTACATTGGAAGGCT45930.12225166648744586No Hit
TGCAATGACTCCGTAA45080.11998922545730588No Hit
CCGTTACTGTTGGTTG44590.11868499474581341No Hit
AATTCTGCTTAGCGAG44300.11791310310023626No Hit
AACTACGACGCTGTTA44280.11785986919364473No Hit
TCCTCATCTTTCTGGA43490.11575712988327934No Hit
TGTCTTCCTGAAATCA42420.11290911588063256No Hit
GGACCACTGGCTAGAG42420.11290911588063256No Hit
CCCACCTCTTGACCTA42310.11261632939437913No Hit
GCGTTTAGAAGTCTAC42280.11253647853449185No Hit
GGCTTAAACTGCATCA42180.1122703090015342No Hit
CCTGAGGACTGACTCA40420.10758572522147967No Hit
TCGTAGAACCGCACTG40190.10697353529567709No Hit
ATGGCCGTGGGCCAAA40060.10662751490283216No Hit
AGGTGATCTGTTAGTC39990.10644119622976181No Hit
ACAAGACCTAAGCTAA39630.10548298591111428No Hit
ATAGCCAACATCTGTG39610.10542975200452276No Hit
TATGGGTACCCTTTGC39480.10508373161167782No Hit
TCCGTAAGATTACGCA38170.10159691072993268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)