FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16130504
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG7300.010.5459545
TATACTG19700.09.1657815
CTAGGAC23950.08.7081673
TAGACTG20500.08.6032525
TAGACAG26350.08.4462225
TAGGACC16250.08.3553454
CTAATAC18350.08.2382263
GTAGGAC14350.08.1935743
AGGACCG19200.08.1651245
GTATTAG18500.08.0976511
TAGGACT22600.07.8657714
CTATACT19550.07.80415254
CTAGTAC9650.07.6876673
GTATAGA15000.07.6536671
CCTACAC24800.07.61952833
TACACTG26900.07.59707835
CGTCAGA25300.07.58135841
GTACTAG12300.07.5125231
CTTAGAC14550.07.50373743
GTGTAAG21850.07.4968951
Produced by FastQC (version 0.11.5)