FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071170
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT396149597.30605698116266No Hit
CTAGCTGA225560.5540422040838383No Hit
CTAGCAGT85440.20986595990833104No Hit
CAAGCTGT72000.17685333700140254No Hit
CTAGATGT69570.17088453687760521No Hit
CTAGCTAT62630.1538378402277478RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCTCT60010.14740234379797454No Hit
CTACCTGT56450.1386579288017941No Hit
CTAGCTGG55160.1354893065138523No Hit
CTAGCGGT54960.13499804724440395No Hit
CTCGCTGT50200.12330607663153344No Hit
ATAGCTGT49700.12207792845791259No Hit
CTAGCTTT41220.10124853543330295No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)