FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004948150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426603
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC27600.6469715402845269No Hit
CTCCTAGTGAACGTAA21630.5070287832012433No Hit
TAGCTTTACCCTATCC19880.46600703698755047No Hit
CGACCAGACGCGCTGT18440.4322520001031404No Hit
CCAGGACTGTAACTCG14960.35067732763248266No Hit
GTTCTCGACCGTAGAG14540.3408321085411964No Hit
ATCTGTACTGGGACTA14260.3342686291470055No Hit
CGATCAAACCCGGCTA13520.3169222907480726No Hit
CTTCCTTTGGCTCAGC13070.3063738417216944No Hit
GGGGGGGGGGGGGGGG12800.3000447723058675No Hit
TGCTGCTACTTTCTTG11140.26113271589745035No Hit
ACAGCCGACTTGCGTA10800.25316277663307574No Hit
GGCAACAGAATAGCAA9750.22854972890486003No Hit
CGCGTTGGAGCGGTTC8970.2102657505924712No Hit
AACTTACCTCACCTAA8900.2086248807439235No Hit
ATCGAAAGATTTCCGC8660.2029990412631885No Hit
GTAACTGTGTGAAAGT8430.19760761176081743No Hit
AGTGGAGTGAGTTGAA8260.19362264212863012No Hit
CCCTGAACTAGCTTTG8070.1891688525397149No Hit
AAGAACCCTTAACCCA7530.17651071370806112No Hit
CAATTCGGATAACGGG7520.17627630372969716No Hit
GGGTAGAACCAGTCTA7190.1685407744436865No Hit
TTCCTAAACTAATGCC7100.1664310846384109No Hit
GTGAAGCGATCCCAGG6980.16361816489804337No Hit
TTTAGTCTGAGCAATA6940.16268052498458754No Hit
TGGAACACTCCTAGTA6690.15682027552548855No Hit
CGCGATCGATATCTGG6660.15611704559039669No Hit
TGCAGATACTGCCACG6560.1537729458067571No Hit
TCCGGGAACGTTCTCC6510.1526008959149373No Hit
CTTTAACTGTCCGTAA6340.14861592628275No Hit
GCAACTTGAAGGTGCA6240.1462718264991104No Hit
CTATTGGGACTTGTGA6240.1462718264991104No Hit
CATAGGTACAAGGTGC6170.1446309566505627No Hit
CACATCCTGAGCCTAT6060.14205244688855914No Hit
AGTCCTGCTCGAACGA6050.14181803691019518No Hit
TCTCAGAGATGCATGT5980.14017716706164748No Hit
TGATAGAGAGACTGTT5960.13970834710491956No Hit
GAGTGTTACACCTGCG5930.1390051171698277No Hit
TGCAGTATGACACCGC5860.13736424732127997No Hit
AGGTGTGGATCATGTG5860.13736424732127997No Hit
CTTAGATCTTGTCACA5840.13689542736455204No Hit
TACGTTTACTGCACCG5840.13689542736455204No Hit
ATGTACGTGTACTTTC5740.13455132758091246No Hit
TGGCAATTGAGTGGTG5660.1326760477540008No Hit
AGAGTACCTTAATCGC5650.13244163777563683No Hit
CGAGTCTCTGCTACGC5510.12915989807854142No Hit
CATTCTATGCCTAGCG5440.1275190282299937No Hit
TTCTTAGACGTCCCGA5440.1275190282299937No Hit
GGTCTAAACTCCTACC5430.12728461825162973No Hit
TGAGGGATGTACGTTG5400.12658138831653787No Hit
TCAAAGCACTCTGCAA5380.12611256835980994No Hit
GCATGATACTTGCGTA5360.125643748403082No Hit
ATTTCTGACGGGTAGT5320.1247061084896262No Hit
AGACTACTGGCCCTAG5160.12095554883580284No Hit
TCTATGGGATGCTCAA5110.11978349894398305No Hit
TGAACTGTGAACGTAA5100.1195490889656191No Hit
AGACCATTGAATCCAC5080.11908026900889117No Hit
CTACAACGAATCTGTG5080.11908026900889117No Hit
GGGTGTGCTCGCAAAG5050.11837703907379929No Hit
TTGCGGTCTTTTGGGT5050.11837703907379929No Hit
CTAGCTCACGAATAGT4960.11626734926852367No Hit
TCCGGGTGAACACCTA4940.11579852931179575No Hit
TCGGACCGATAGGCGA4920.11532970935506782No Hit
TACTGTTTGCTTAAGC4860.11392324948488407No Hit
GGTCGCATGGTACTAC4840.11345442952815615No Hit
CTTTCGTCTCGAACGA4810.11275119959306427No Hit
TCCATTTGAAATCTGC4810.11275119959306427No Hit
GGGATGTGACTGGAAG4770.11181355967960845No Hit
CACCAGGTGCGCCACA4730.1108759197661526No Hit
CGAGTCTCTTGGAACG4730.1108759197661526No Hit
GGCTGACTGAAACTGA4710.1104070998094247No Hit
CTAACAGTGCATGATC4690.10993827985269677No Hit
CACATTACTGATGGAC4680.1097038698743328No Hit
GTAATCTCTACACAAA4620.10829741000414905No Hit
AGCCAATTGTTATGGC4600.10782859004742115No Hit
GTATTCACTGCTCCTT4570.10712536011232925No Hit
TGCTAGTGAAGGCGTA4560.1068909501339653No Hit
GTGTGATACCGTGCAC4490.1052500802854176No Hit
ATGTCCAGACCGCTAG4470.10478126032868967No Hit
AGGCGATGACCTGCGA4460.10454685035032572No Hit
TGTAAGCACGCGCTGT4460.10454685035032572No Hit
AAAGCCTTGAACCAAA4460.10454685035032572No Hit
AGCTTGTGATTGCGTA4450.10431244037196175No Hit
ACCTCAGCTAATCCGT4440.1040780303935978No Hit
CTCAATTTGGAATCTC4430.10384362041523383No Hit
ACGAGGATGAACTAGA4420.10360921043686988No Hit
TCGTTGCTGTATTGCG4410.10337480045850593No Hit
TAGTACCGAACGAGCA4400.10314039048014195No Hit
CATAGTCGAAGATTCC4380.10267157052341405No Hit
TCATCATTGGACTCGC4380.10267157052341405No Hit
GTCTAATCTAAGTTCC4350.10196834058832217No Hit
GATATGAGACCAACGC4320.10126511065323028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)