FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665716
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG359022697.94064788434238No Hit
TAATGGTG90540.24699131083804637No Hit
TATTGGTA83260.22713161630633685No Hit
AATTGGTG66130.18040131859642156No Hit
TATTGGAG51460.14038185173101245No Hit
TATTGGGG50210.1369718767083975No Hit
TATTGGTC50200.13694459690821656No Hit
TATTGCTG48080.13116127926986162No Hit
TATAGGTG46490.1268237910410954RNA PCR Primer, Index 39 (100% over 8bp)
TATTCGTG44800.12221350481051996No Hit
TATTGGTT43570.11885808938826685No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)