FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800954
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT490021.749475357324683No Hit
GAAGCAGTGGAACCTT166530.5945474291973378No Hit
GTTCTTCCTAACGTAA145070.5179306764766576No Hit
GATACGTACCGCTCGA142990.5105046352064333No Hit
CGCTTAAGATAATAGG113530.40532618529258246No Hit
CATGGAGGATCGACAT111460.3979358461438496No Hit
TGTCCCATGAGACAAT111000.3962935485552423No Hit
AAAGCATGATCTTTGT102340.36537551134363505No Hit
TGAGCGCACAGGACAC100930.36034151221333877No Hit
TGTCGAAACTTCGCAA95010.33920585629039246No Hit
TAATTGCCTGTCTGGC94160.3361711759636181No Hit
GGGGGGGGGGGGGGGG79660.2844030998009964No Hit
GGAAGGACTAGGTAAT79580.2841174828290647No Hit
AATGATGGAAAGCTAA73660.26298182690611843No Hit
CATGTCAGAGGTTTGT72820.2599828487008355No Hit
TCGACCTTGTTTCGCC71450.255091658056505No Hit
ATGGGTACTTGGCTGC56810.20282375219300283No Hit
GCTATGATGACCTGCG56760.20264524158554548No Hit
AAACGACCTAGGGAGG54460.19443375364250895No Hit
AGCCTACCTTAACTCG52990.18918554178326386No Hit
TTGCGATGACATTCAG52570.18768605268062238No Hit
CAGCTCACTGTTTACG51810.1849726914472712No Hit
TGTTCCTCTTTATGGC51160.18265205355032607No Hit
CTGCCTTGACCATAAC48310.17247694892525903No Hit
GTGAGATCTCGATTAC47390.16919235374804442No Hit
CCTGTTGACCCTAGTA47200.1685140134397066No Hit
CACAGCTCTGGACTGA46460.16587205644933833No Hit
CAATGTGACCAACGCC46220.1650152055335432No Hit
TAACCAGACATCACAC46100.16458678007564564No Hit
AGCTTGTGACTCACTC45550.16262316339361518No Hit
CATGCAAACGAAGGCT45110.16105227004799078No Hit
CATGATGTGTCATGTG44260.15801758972121643No Hit
CTATTCAACCGAACGA44020.1571607388054213No Hit
ATGGGTACTTTCGGGC42310.15105567603038106No Hit
ATGCCGCGATGCCACG41840.14937767632028232No Hit
TGGGCCATGCGTTCTC41770.14912776146984208No Hit
ATTAGCGTGGTCGGTC41230.14719984690930304No Hit
CATTAGTCTATTCTGG40650.14512912386279817No Hit
AGAACGCGAGGAATAT40350.14405806021805428No Hit
GGGCTGACTAATACAG40300.14387954961059696No Hit
CCGCTATTGTTCGCAA40180.1434511241526994No Hit
TTGTTTGTGATACGCT40060.14302269869480186No Hit
GCATCTCTGAGCCTAT39570.14127329474172015No Hit
GGGTCGTACCGCTCGA39120.1396666992746043No Hit
GCAGATACTCGGCTCA38130.13613218924694942No Hit
CACTATACTACGCCAC38040.13581087015352628No Hit
GGCTTGCGAGTACGTT37560.13409716832193602No Hit
AGCTCGGACCTTAAGC37290.1331332110416665No Hit
AACCAACACCGGAATG37130.1325619770978031No Hit
TTGACGTGACCTCAAA36620.13074116890173848No Hit
AACGTCCCTTACAGAA36470.13020563707936653No Hit
GGTAGCCTGTGCCACG36370.1298486158644519No Hit
ATAGGAAACAGACTTC36350.129777211621469No Hit
CCCAATGCTGGAATGC36290.12956299889252018No Hit
TGGAGTTACAAGTCTC36190.12920597767760555No Hit
GATACCATGTCTGGAT36000.12852763736926776No Hit
CTGCCATTGCACAGTT35830.1279207013039129No Hit
CCTACGGGATGGAACG34650.12370785096792022No Hit
CGATCAAACCGATCGC34600.1235293403604629No Hit
ATAGCCAACATGGCCC34480.12310091490256535No Hit
TTGTAACCTCATCCTC34420.12288670217361657No Hit
CTGATTACTGCTTTGA33960.12124440458500924No Hit
ATCTTACTGCGCACTG33800.12067317064114584No Hit
TCAGGCTCTTAAAGCA33580.11988772396833364No Hit
TGTTTGTTGCGCTCGA33540.11974491548236779No Hit
GGGATCAGATGAGTAA33400.1192450857814873No Hit
TCCAAAGTGAAGGCCA33040.11795980940779464No Hit
TGGGTGCACTTCTGAG32630.11649602242664464No Hit
CGCTTCCCTGCGCTGT32340.11546066090339221No Hit
CGCTTACTGGCCACGT32330.11542495878190075No Hit
CGCTGAGTGTTAGCTG32160.11481802271654587No Hit
TTCTAACACTACGTTG32110.11463951210908856No Hit
CTCGGATCTTTCTGAG31690.11314002300644709No Hit
CATGTTACTAACGTGG31350.11192615087573733No Hit
TACAACCGATTCCATT31050.11085508723099344No Hit
AGCCGGTACGCGCAAA30830.11006964055818125No Hit
CTTAGGCCTTATGACC30780.10989112995072392No Hit
AACCACTGAAAACATG30730.1097126193432666No Hit
GCATAGTACGCTCCTT30700.10960551297879223No Hit
TACGCCTTGGGTTGAG30680.10953410873580931No Hit
AGTGGTACTCAACGCC30660.10946270449282637No Hit
CCTCCCTTGTTTCGCC30390.10849874721255687No Hit
ACCTGCTTGCCAAACT30350.10835593872659101No Hit
GTCCTGACTTTACGAC30200.10782040690421907No Hit
GTAAGAAACGACAAAT29990.10707066235289833No Hit
CCTTCAGTGTAGCTTG29880.10667793901649224No Hit
ATTGAGAGATGATCAC29780.1063209178015776No Hit
TTCACGCTGTGGGCAA29650.10585679022218858No Hit
AACAGCTACTCGAGAG29640.10582108810069711No Hit
TGAGATAGACTCATCC29550.10549976900727394No Hit
TTCTATGACCAACGCC29490.10528555627832517No Hit
GTGTACTGAATCACAC29390.10492853506341053No Hit
TATCTCCCTGGAATTA29120.10396457778314103No Hit
AGAACAATGAGTCGGA29060.10375036505419226No Hit
GTTGCTAGACGCTCGA29060.10375036505419226No Hit
CGAGTCTCTTACTTTC29000.10353615232524346No Hit
GCATGGGCTATGTGGG28850.10300062050287151No Hit
ACCTCCATGGAATTGA28770.1027150035309398No Hit
ATCGCAATGTAACTCG28570.10200096110111055No Hit
AGGTTACTGGTCCCGA28280.10096559957785811No Hit
GCATATCGAGCCGCTA28240.10082279109189225No Hit
TGGTCCTTGTTTCTGT28080.10025155714802884No Hit
AGAACATGAAAGCTAA28030.10007304654057153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)