FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735670
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG266493197.41419834994718No Hit
GAGAAGGA167640.6127932097073112No Hit
GAGCAGGG144110.5267813734843749No Hit
GAGAAGTG42030.1536369518253298No Hit
GAGATGGG37500.13707793703187884No Hit
GAGAAGGC37220.13605442176870747TruSeq Adapter, Index 20 (100% over 8bp)
GAGAAGCG32970.12051892223842788No Hit
GAGAACGG32030.11708283528349545No Hit
GTGAAGGG28690.10487376035852276No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)