FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942551
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT400931.0169304087632602No Hit
TGCTGCTACCGTTCTC378070.9589476458262682No Hit
AAACGACCTCTTCAAG375960.9535957810057498No Hit
ATGGGTTACAATCGAA364980.9257457925084546No Hit
TATGCTTTGGACTGTT357910.9078132407164803No Hit
GCCTCTTGATCTGTGA343460.8711618441968157No Hit
AAAGCGGACTCGGGCT330760.8389491981207092No Hit
TCCAAAGTGCTCACGG313840.7960328224035655No Hit
TAGTGCATGAACCCGC305650.7752594703277143No Hit
TAACTCACTGGTGTCC290570.7370101236483688No Hit
GCAGGTCTGGCTGTTA284790.722349565040503No Hit
TGTGGCTCTTACGATC282850.7174288931202157No Hit
GAAGTATGAACAGGTG280830.7123053068939375No Hit
TTCCCATCTCTTTGTT253940.6441007357926378No Hit
TCACCTGACTCAATGC241320.6120910040225225No Hit
GGAACTACTACCAAGG238670.6053694676365632No Hit
GAGCCTGACCGAGTTT238530.6050143676010785No Hit
TGAGAATGAGCAGTTT235600.5975826311441501No Hit
ATGTCCAGAGTCGGTC234490.5947671951485218No Hit
CTAGAGACTAATCTGC232590.5899479803812303No Hit
TCGTATACTGCTAATC224510.5694536354761168No Hit
GAAGTAATGAGTCTGT221020.5606014988772499No Hit
TTCGTCCGACCTGTGG219890.5577353343051238No Hit
TGCGGTGACCTTAGTG218580.5544126125445175No Hit
ATTCCTAACTGCTACA213950.5426689470852756No Hit
TCACACAGATTGGGAG210520.5339689962159018No Hit
ACTGATTCTCGGCTCA207620.5266133526237201No Hit
TCCGCGAGAGAACAGC204990.5199425448142586No Hit
AGGTCGAGAAGGGAGG204590.5189279732843025No Hit
TGGCCTGGACCCGAGT203590.5163915444594122No Hit
TGGACCTCTAGGGAGG195710.49640448531927683No Hit
CCATGATGATTCGGGC190710.4837223411948254No Hit
ACGAGGATGGAGCTGA175650.4455237230919778No Hit
GCAATCATGGAACGTG174440.4424546442138605No Hit
TTCGATGGAAATCCTG172140.4366208579166129No Hit
AAGGTGCGAACAGATA168450.42726143555276774No Hit
TCCCAGACTGTCTTGA164770.4179273774771715No Hit
TTTCGTAACAGTACGG162200.41140875539720345No Hit
TGGTCCTTGGAGAGGG157250.39885343271399665No Hit
TCTCTCTACTCTGGAT154190.39109196050983236No Hit
GCGGTTCGAGTACGTT153300.38883453885568003No Hit
TCCTTCACTTGTCACA139630.35416155681942985No Hit
TCGCCGAGAAGGTAAT137630.3490886991696493No Hit
AGCCCAGTGAAAGTAC137400.34850532053992456No Hit
TTCGATGGACAGGTGA134970.34234179849544116No Hit
TGATATCCTCGATGTT132580.33627973360395336No Hit
AGCTGCTCTTGTCACA131720.3340984048145478No Hit
CTTTCGGTGCTTTGTT131440.3333882047435785No Hit
AACTCCCTGCTTCACC130800.3317648902956487No Hit
CCCTTGCCTCCTACGG129200.3277066041758242No Hit
AACCCTCACGCGGTTC126560.32101043207811386No Hit
ATGGCCGTGATACGCT123380.3129445884149628No Hit
TCGGCTTACCTCCTAC119910.3041431803925935No Hit
TGTTTGTTGCAAGTTC116530.29557005096446437No Hit
GGGGGGGGGGGGGGGG116110.2945047508580105No Hit
CTAACTGGAACCTTGA114970.29161322199763556No Hit
AAACACGCTGCAGTTT113600.28813831450753585No Hit
AAATAGGACTCTATCT111820.28362347119923115No Hit
ACCCTTGTGGGCCATC111490.2827864496870174No Hit
TGGCCTTTGCGAAAGC110810.281061678086092No Hit
TACAACGTGAGCAGGT109000.27647074191304055No Hit
CAATGTGTGGTTGACT106000.2688614554383697No Hit
TCAAAGGCTCTTAGTG103350.26213991905241046No Hit
CTTTCGGTGAGCCATT103070.26142971898144124No Hit
TGAACTGTGTGCTGGC98000.2485700248392475No Hit
AGTGTTCGATTCGCCT94910.24073245977033653No Hit
CAGCCGATGTAATAGG94550.23981934539337602No Hit
GAGGTTGGAGGAATAT86460.21929963620001364No Hit
ACAGCCGACAAACATG82860.21016849243040864No Hit
CGACGTTCTTGCTACA82020.20803789221750082No Hit
GTGCTAATGGGAGTCA81210.20598338486933968No Hit
GGCTTGCGAATACGCT81050.20557755625735724No Hit
GATAAGAACATTGGTC80520.20423324898016537No Hit
TTGGGAGGAATCTGTG79480.20159536300227948No Hit
TGTACTGGAAATGCTC79260.2010373486608036No Hit
GTAAAGCCTGAAATCA78510.19913502704213593No Hit
AGGGACCCTAATCCTG73790.1871630829886538No Hit
TCTTGAACTCGTACAT72190.18310479686882933No Hit
TAACGAGCTTACTTTC72160.18302870400408264No Hit
GGGTGAAACCGACTAT71010.1801118108554588No Hit
CGGATGAGAACAGGCA70930.1799088965494676No Hit
TACCACCTGAAACATG69090.17524186751166948No Hit
GGACCACTGGCTAGAG69000.17501358891742935No Hit
GGAACATCTTTACGAC68940.17486140318793592No Hit
CATGATGTGCCTAGTA67080.17014364557364No Hit
AGCTTGTGAACCGTGC67020.1699914598441466No Hit
TACGTGTGAAACGCAG66720.16923053119667952No Hit
AACTAGCACTGCCACG65130.16519760936510397No Hit
ACGTGTTGATCTTTGT63850.16195098046924442No Hit
ACTTATCTGTCAGGTT63620.16136760183951965No Hit
GGACATTTGGCCGCTA63080.15999793027407888No Hit
TCGGACCGAATGTCCA60270.1528705652761372No Hit
AACTACGACATTGCCA59930.1520081794756745No Hit
TGGTAGGGATGTGCTA59540.1510189722339673No Hit
CTCGGATCTAGTACGG58910.14942102207428642No Hit
GTCCGAGTGGTCTTGA58600.14863472913857043No Hit
CGTCGTACTTGCGCCA57620.14614902889017797No Hit
GTAGATAGAGCTGAGA57600.14609830031368015No Hit
AACGAACCTGACACCC56620.1436126000652877No Hit
TACCACCTGTACTTTC55250.140137692575188No Hit
GGTAGTTTGTCCAGCG55230.1400869639986902No Hit
AGTTTGCGAGCAGGAT55170.13993477826919676No Hit
CCTTCAGTGCAACGCC54970.1394274925042187No Hit
TTTAGGGACTGGGCAA53980.13691642796757733No Hit
TGAGGAGTGGTCCCAG53820.1365105993555949No Hit
CTTTAACTGTTCGCCT53570.13587649214937234No Hit
GCCATAATGACCGTTA51210.12989052012263128No Hit
AGAACGCGACACAGTT49830.12639024834428267No Hit
CGATCGGTGCGAGGAT48960.12418355526662814No Hit
GTCCGTTACGGATGGT48480.12296606943068081No Hit
CAGGCCATGTCGTAGT47810.1212666621180043No Hit
CTTACATTGGAAGGCT47780.12119056925325761No Hit
CCGTTACTGTTGGTTG46930.11903460475210086No Hit
TGCAATGACTCCGTAA46850.11883169044610964No Hit
GAGAAGAGATGCTACA46500.11794394035739804No Hit
GGGATGTGATGCATGT45650.11578797585624132No Hit
AACTACGACGCTGTTA45300.1149002257675297No Hit
GAACCATACTTTGGGT45220.11469731146153848No Hit
CAAAGACCTGCTTTGA45140.11449439715554727No Hit
TCCTCATCTTTCTGGA44800.11363201135508458No Hit
GAAAGTCCTTATCTGG44750.11350518991384004No Hit
GAAGGACACCCAGCTG43480.1102839253062294No Hit
CCCACCTCTTGACCTA43430.11015710386498487No Hit
AATTCTGCTTAGCGAG42840.1086606108582996No Hit
GCTGGACACATTGGTC42460.10769676790484131No Hit
ATGGCCGTGGGCCAAA42250.10716411785161434No Hit
ACAAGACCTAAGCTAA42160.10693583925737422No Hit
CCTGAGGACTGACTCA41830.10609881774516043No Hit
TGTCTTCCTGAAATCA41300.10475451046796859No Hit
ATAGCCAACATCTGTG41150.10437404614423502No Hit
TCGTAGAACCGCACTG41020.10404431039699931No Hit
GGCTTAAACTGCATCA40780.10343556747902563No Hit
TATGGGTACCCTTTGC40560.10287755313754976No Hit
TCCGTAAGATTACGCA40340.1023195387960739No Hit
AGGTGATCTGTTAGTC39940.1013049672661178No Hit
GCGTTTAGAAGTCTAC39550.10031576002441059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)