FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3982499
Sequences flagged as poor quality0
Sequence length8
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTTGAT389904497.9044564731843No Hit
AAGTTGAA166080.4170245868234996No Hit
AAGTTCAT76260.19148780702769794No Hit
AACTTGAT70350.1766478786309802No Hit
AATTTGAT70340.1766227687690568No Hit
AAGTAGAT68680.17245453168977568No Hit
AAGATGAT66560.16713124096201906No Hit
AAGTTGCT57590.14460769481674698No Hit
AAGTTTAT47390.1189956356548991No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)