FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713185
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT394531.0625110249018026No Hit
TGCTGCTACCGTTCTC364650.9820410240804054No Hit
AAACGACCTCTTCAAG360130.9698681859374095No Hit
ATGGGTTACAATCGAA352980.9506124795828917No Hit
TATGCTTTGGACTGTT350270.943314162908662No Hit
GCCTCTTGATCTGTGA330270.8894520472316891No Hit
AAAGCGGACTCGGGCT323000.8698731681831097No Hit
TCCAAAGTGCTCACGG307600.8283993391118407No Hit
GCAGGTCTGGCTGTTA292150.7867908547513793No Hit
TAGTGCATGAACCCGC287820.7751297067073146No Hit
GAAGTATGAACAGGTG276590.7448861287546944No Hit
TGTGGCTCTTACGATC271660.7316091172403206No Hit
TAACTCACTGGTGTCC269780.7265460783666853No Hit
TTCCCATCTCTTTGTT245030.6598917102164314No Hit
TCACCTGACTCAATGC232490.6261201636869695No Hit
GAGCCTGACCGAGTTT231080.622322884531743No Hit
TGAGAATGAGCAGTTT228540.6154823958407675No Hit
GGAACTACTACCAAGG228230.6146475330477743No Hit
CTAGAGACTAATCTGC214710.5782367428501408No Hit
TCGTATACTGCTAATC213110.573927773595983No Hit
GAAGTAATGAGTCTGT212700.572823600224605No Hit
ATGTCCAGAGTCGGTC212520.5723388411835123No Hit
TGCGGTGACCTTAGTG208760.5622127634362415No Hit
ATTCCTAACTGCTACA206060.5549413778198501No Hit
TCACACAGATTGGGAG205760.5541334460846955No Hit
TTCGTCCGACCTGTGG205280.5528407553084482No Hit
AGGTCGAGAAGGGAGG202710.5459194734439571No Hit
ACTGATTCTCGGCTCA199380.5369514311837411No Hit
TCCGCGAGAGAACAGC196890.5302455977819581No Hit
TGGACCTCTAGGGAGG190750.5137099282691274No Hit
TGGCCTGGACCCGAGT189900.5114207883528561No Hit
CCATGATGATTCGGGC185380.49924795020986024No Hit
GCAATCATGGAACGTG171790.46264864260735733No Hit
TTCGATGGAAATCCTG169650.4568853962299212No Hit
ACGAGGATGGAGCTGA167290.4505296665800384No Hit
AAGGTGCGAACAGATA161330.43447875610830056No Hit
TTTCGTAACAGTACGG157800.42497209269131486No Hit
TCCCAGACTGTCTTGA155260.4181316040003393No Hit
TGGTCCTTGGAGAGGG153110.4123414265650648No Hit
TCTCTCTACTCTGGAT149340.4021884177599554No Hit
GCGGTTCGAGTACGTT142450.3836329189092383No Hit
TTCGATGGACAGGTGA135240.36421562620768966No Hit
TCGCCGAGAAGGTAAT133850.36047220916814005No Hit
TGATATCCTCGATGTT133210.3587486214664769No Hit
TCCTTCACTTGTCACA132520.35689037847562133No Hit
AGCCCAGTGAAAGTAC130690.3519619948911783No Hit
AGCTGCTCTTGTCACA128350.3456601273569725No Hit
CTTTCGGTGCTTTGTT128000.3447175403326255No Hit
AACTCCCTGCTTCACC126970.3419436413752614No Hit
CCCTTGCCTCCTACGG120790.32530024763107684No Hit
AACCCTCACGCGGTTC120240.32381903944996004No Hit
GGGGGGGGGGGGGGGG120010.32319962511967487No Hit
ATGGCCGTGATACGCT118830.3200217602947335No Hit
TCGGCTTACCTCCTAC116180.3128850299675346No Hit
TGTTTGTTGCAAGTTC110650.29799215498285164No Hit
AAACACGCTGCAGTTT110070.29643015362821945No Hit
ACCCTTGTGGGCCATC107810.2903437345567215No Hit
CTAACTGGAACCTTGA106060.28563079943498637No Hit
TGGCCTTTGCGAAAGC105570.2843111776009006No Hit
TACAACGTGAGCAGGT102580.27625879130719316No Hit
CAATGTGTGGTTGACT101250.2726769606146745No Hit
AAATAGGACTCTATCT101050.2721383394579047No Hit
TCAAAGGCTCTTAGTG97110.2615275026695411No Hit
CTTTCGGTGAGCCATT96440.2597231217943625No Hit
TGAACTGTGTGCTGGC93610.25210163242607087No Hit
AGTGTTCGATTCGCCT88750.2390131383165665No Hit
CAGCCGATGTAATAGG87190.23481189329376262No Hit
GAGGTTGGAGGAATAT86590.23319602982345344No Hit
GGCTTGCGAATACGCT80600.21706432617820012No Hit
GATAAGAACATTGGTC78310.21089711393318675No Hit
ACAGCCGACAAACATG78180.2105470101812864No Hit
CGACGTTCTTGCTACA78070.21025076854506308No Hit
GTGCTAATGGGAGTCA76110.20497228120871974No Hit
GTAAAGCCTGAAATCA75630.20367959043247236No Hit
TGTACTGGAAATGCTC74470.20055558772320795No Hit
TTGGGAGGAATCTGTG74090.19953220752534548No Hit
AGGGACCCTAATCCTG70870.19086040690135286No Hit
GGACATTTGGCCGCTA68420.1842622977309237No Hit
GGGTGAAACCGACTAT67720.18237712368222966No Hit
TAACGAGCTTACTTTC66770.17981867318757347No Hit
TCTTGAACTCGTACAT66430.17890301722106494No Hit
GGAACATCTTTACGAC66390.17879529298971097No Hit
CGGATGAGAACAGGCA66060.17790656808104094No Hit
GGACCACTGGCTAGAG65770.17712556740372484No Hit
TACGTGTGAAACGCAG65610.17669467047830906No Hit
TACCACCTGAAACATG65600.17666773942047057No Hit
AGCTTGTGAACCGTGC64730.17432473738852225No Hit
ACTTATCTGTCAGGTT63020.1697195264981411No Hit
CATGATGTGCCTAGTA62610.16861535312676312No Hit
AACTAGCACTGCCACG60650.1633368657904198No Hit
ACGTGTTGATCTTTGT58000.15620013546322092No Hit
TGGTAGGGATGTGCTA57110.15380327131559562No Hit
AACTACGACATTGCCA56320.15167571774635522No Hit
CGTCGTACTTGCGCCA56180.1512986829366164No Hit
TCGGACCGAATGTCCA55650.14987133687117662No Hit
AACGAACCTGACACCC53990.1454007812699879No Hit
TACCACCTGTACTTTC53120.14305777923803958No Hit
CTCGGATCTAGTACGG53010.14276153760181623No Hit
GTCCGAGTGGTCTTGA52950.1425999512547853No Hit
GTAGATAGAGCTGAGA52860.14235757173423894No Hit
CCTTCAGTGCAACGCC52110.14033774239635247No Hit
GGTAGTTTGTCCAGCG51990.14001456970229062No Hit
TTTAGGGACTGGGCAA51170.13780622295953474No Hit
AGTTTGCGAGCAGGAT51160.13777929190169624No Hit
TGAGGAGTGGTCCCAG50540.13610956631571008No Hit
CTTTAACTGTTCGCCT49730.13392815063079272No Hit
GCCATAATGACCGTTA49400.13303942572212266No Hit
AGAACGCGACACAGTT47130.12692607559278626No Hit
CTTACATTGGAAGGCT46250.12455614250299946No Hit
GAGAAGAGATGCTACA45150.12159372614076594No Hit
GTCCGTTACGGATGGT45070.12137827767805805No Hit
GAACCATACTTTGGGT45050.12132441556238108No Hit
GGGATGTGATGCATGT44720.12043569065371103No Hit
CCGTTACTGTTGGTTG43820.11801189544824726No Hit
TGCAATGACTCCGTAA43450.11701544630822326No Hit
GAAAGTCCTTATCTGG43420.1169346531347078No Hit
AACTACGACGCTGTTA42630.11480709956546739No Hit
CAGGCCATGTCGTAGT42570.11464551321843645No Hit
GAAGGACACCCAGCTG42370.11410689206166674No Hit
TCCTCATCTTTCTGGA42030.11319123609515822No Hit
CGATCGGTGCGAGGAT42020.11316430503731971No Hit
AATTCTGCTTAGCGAG41810.1125987528227115No Hit
GCTGGACACATTGGTC41580.1119793384924263No Hit
CAAAGACCTGCTTTGA40660.10950168117128556No Hit
GACGAGTGATGCTACA40100.10799354193233032No Hit
CCCACCTCTTGACCTA40020.10777809346962244No Hit
ATGGCCGTGGGCCAAA39960.10761650712259152No Hit
GGCTTAAACTGCATCA39890.10742798971772212No Hit
TGTCTTCCTGAAATCA39880.10740105865988364No Hit
CCTGAGGACTGACTCA39580.10659312692472905No Hit
TCGTAGAACCGCACTG39170.1054889535533511No Hit
ATAGCCAACATCTGTG38800.1044925044133271No Hit
ACAAGACCTAAGCTAA38560.10384615902520344No Hit
GCGTTTAGAAGTCTAC38270.10306515834788733No Hit
TATGGGTACCCTTTGC38250.10301129623221034No Hit
AGGTGATCTGTTAGTC37140.10002194881213837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)