FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12982246
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA1272307798.00366592960879No Hit
GTGGCGCA568840.4381676329350099No Hit
GTGGAGCC242320.1866549131791217No Hit
GTGGAGCT187830.14468220676144944No Hit
GAGGAGCA163940.12628015214008426No Hit
GTGTAGCA152250.11727554692770419No Hit
GTGGACCA140770.10843270109039685ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)