FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667396
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC261285397.95519675368787Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGATA118750.44519074033251904No Hit
TGTAGCTC97110.3640629287889762No Hit
TGTAGAAC38220.1432858113306011Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC34860.13068925648835045No Hit
AGTAGATC34700.13008942054348135No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)