FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752555
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT360520296.07326208410004No Hit
CTTTCCTA680651.8138308432521308No Hit
CTTTCCAT104440.2783170399900867No Hit
CTTTACTT83540.22262165377989132No Hit
CTTTCATT81100.21611941730367712No Hit
CATTCCTT70070.18672611060997107TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT68150.18160959666147466No Hit
ATTTCCTT61100.16282239700683934No Hit
CTTACCTT48890.1302845661156199No Hit
CGTTCCTT42710.11381578684389701No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)