FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4068344
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCTACCGTTCTC401270.986322690509947No Hit
GCACTGTCTCTTTGTT398610.979784403678745No Hit
AAACGACCTCTTCAAG394570.9698540733035358No Hit
ATGGGTTACAATCGAA374200.9197845609909093No Hit
TATGCTTTGGACTGTT372000.914376955341043No Hit
AAAGCGGACTCGGGCT347630.8544754327559322No Hit
GCCTCTTGATCTGTGA340060.8358683533152556No Hit
TCCAAAGTGCTCACGG328710.8079700241670812No Hit
GCAGGTCTGGCTGTTA327530.8050695811366984No Hit
TAGTGCATGAACCCGC319780.78602006123376No Hit
TAACTCACTGGTGTCC304250.7478472813508396No Hit
TGTGGCTCTTACGATC297590.7314769842471531No Hit
GAAGTATGAACAGGTG281380.6916327626179104No Hit
TTCCCATCTCTTTGTT260660.6407029494064415No Hit
TCACCTGACTCAATGC254370.62524211325296No Hit
GGAACTACTACCAAGG247270.6077902950193002No Hit
ATGTCCAGAGTCGGTC244420.6007849877001551No Hit
TGAGAATGAGCAGTTT243410.5983024051063528No Hit
CTAGAGACTAATCTGC236920.582349968439247No Hit
TTCGTCCGACCTGTGG233280.5734028390912863No Hit
TCGTATACTGCTAATC231920.5700599555986416No Hit
GAGCCTGACCGAGTTT231380.5687326342118562No Hit
TGCGGTGACCTTAGTG226960.557868262860761No Hit
GAAGTAATGAGTCTGT226450.5566146815510192No Hit
TCACACAGATTGGGAG226270.5561722410887575No Hit
ATTCCTAACTGCTACA222170.546094430559461No Hit
TCCGCGAGAGAACAGC218800.537810961904893No Hit
ACTGATTCTCGGCTCA215310.5292325329421504No Hit
AGGTCGAGAAGGGAGG212900.5233087467529786No Hit
TGGCCTGGACCCGAGT209370.5146319976875112No Hit
TGGACCTCTAGGGAGG207380.5097405725769502No Hit
CCATGATGATTCGGGC196370.4826779643019371No Hit
GCAATCATGGAACGTG186210.4577046582098269No Hit
ACGAGGATGGAGCTGA182310.44811844819415464No Hit
TTCGATGGAAATCCTG177420.4360988156360426No Hit
AAGGTGCGAACAGATA174870.42983090908733385No Hit
TCCCAGACTGTCTTGA174200.4281840473666927No Hit
TTTCGTAACAGTACGG167320.4112729896980197No Hit
TCTCTCTACTCTGGAT165270.40623408443337145No Hit
TGGTCCTTGGAGAGGG164450.40421852232751215No Hit
TCCTTCACTTGTCACA148040.3638827001846452No Hit
TCGCCGAGAAGGTAAT143920.35375572960398627No Hit
TTCGATGGACAGGTGA143490.3526987884996942No Hit
TGATATCCTCGATGTT141690.34827438387707627No Hit
AGCCCAGTGAAAGTAC141280.3472666028241466No Hit
AGCTGCTCTTGTCACA140620.34564432112918675No Hit
GCGGTTCGAGTACGTT139030.3417360970458742No Hit
AACCCTCACGCGGTTC136340.33512407013762846No Hit
AACTCCCTGCTTCACC134880.3315353863881717No Hit
CTTTCGGTGCTTTGTT133270.32757800225349676No Hit
CCCTTGCCTCCTACGG130150.31990903424095896No Hit
ATGGCCGTGATACGCT129160.3174756116985191No Hit
TCGGCTTACCTCCTAC122310.3006382941068897No Hit
TGTTTGTTGCAAGTTC121950.2997534131823661No Hit
CTAACTGGAACCTTGA119080.29269894581185857No Hit
ACCCTTGTGGGCCATC117520.28886446180558967No Hit
AAACACGCTGCAGTTT117010.28761088049584793No Hit
TGGCCTTTGCGAAAGC115450.28377639648957903No Hit
TACAACGTGAGCAGGT113680.2794257319440047No Hit
AAATAGGACTCTATCT113170.278172150634263No Hit
CAATGTGTGGTTGACT110620.27190424408555425No Hit
TCAAAGGCTCTTAGTG110570.27178134395714815No Hit
GGGGGGGGGGGGGGGG107430.26406321589324794No Hit
ACAGCCGACAAACATG105270.25875393034610644No Hit
CTTTCGGTGAGCCATT103380.2541083054923576No Hit
TGAACTGTGTGCTGGC99610.24484163581054108No Hit
CAGCCGATGTAATAGG99460.24447293542532292No Hit
AGTGTTCGATTCGCCT98230.241449592266534No Hit
GGACATTTGGCCGCTA91920.22593959606168998No Hit
GAGGTTGGAGGAATAT88550.21765612740712192No Hit
TTGGGAGGAATCTGTG84160.20686549613307034No Hit
CGACGTTCTTGCTACA83160.20440749356494928No Hit
TGTACTGGAAATGCTC81550.20045010943027433No Hit
GTGCTAATGGGAGTCA79810.19617318496174363No Hit
TCTTGAACTCGTACAT78390.19268282131501172No Hit
GGAACATCTTTACGAC77770.19115885972277666No Hit
TAACGAGCTTACTTTC75370.18525965355928603No Hit
CGGATGAGAACAGGCA74050.1820150901693662No Hit
AGGGACCCTAATCCTG73190.17990120796078207No Hit
GGACCACTGGCTAGAG72720.17874594675376518No Hit
GGGTGAAACCGACTAT72400.17795938593196642No Hit
TACGTGTGAAACGCAG72040.17707450500744285No Hit
TACCACCTGAAACATG71450.1756242834922514No Hit
CATGATGTGCCTAGTA70120.17235514007665037No Hit
AGCTTGTGAACCGTGC68790.16908599666104931No Hit
TCGGACCGAATGTCCA67490.1658905933224919No Hit
ACGTGTTGATCTTTGT66870.16436663173025684No Hit
ACTTATCTGTCAGGTT65160.16016344733876978No Hit
AACTAGCACTGCCACG64610.1588115459263032No Hit
CTCGGATCTAGTACGG62570.15379722068733617No Hit
TGGTAGGGATGTGCTA62220.15293691978849377No Hit
GTCCGAGTGGTCTTGA62210.15291233976281257No Hit
AACGAACCTGACACCC61610.15143753822193992No Hit
GTAGATAGAGCTGAGA61130.15025769698924182No Hit
AACTACGACATTGCCA61080.15013479686083578No Hit
CCTTCAGTGCAACGCC58840.14462887110824454No Hit
TACCACCTGTACTTTC58700.14428475074870759No Hit
TTTAGGGACTGGGCAA58350.1434244498498652No Hit
CGTCGTACTTGCGCCA58090.14278536918215373No Hit
GGTAGTTTGTCCAGCG57740.14192506828331133No Hit
TGAGGAGTGGTCCCAG57360.14099102730742533No Hit
AGTTTGCGAGCAGGAT56820.13966370592063992No Hit
CTTTAACTGTTCGCCT56230.1382134844054485No Hit
GCCATAATGACCGTTA54550.13408404009100508No Hit
CGATCGGTGCGAGGAT53620.1317980977026525No Hit
AGAACGCGACACAGTT51830.12739827310571575No Hit
TGCAATGACTCCGTAA51620.12688209256641034No Hit
CAGGCCATGTCGTAGT51350.12621843187301762No Hit
CAAAGACCTGCTTTGA51130.12567767130803098No Hit
GGCTTGCGAATACGCT51120.12565309128234975No Hit
CTTACATTGGAAGGCT50880.1250631706660007No Hit
GTCCGTTACGGATGGT50870.1250385906403195No Hit
GGGATGTGATGCATGT49970.12282638832901052No Hit
CCGTTACTGTTGGTTG49130.12076166617178881No Hit
GAGAAGAGATGCTACA47750.11736962262778171No Hit
AACTACGACGCTGTTA46670.11471497985421095No Hit
GAACCATACTTTGGGT46410.11407589918649946No Hit
GAAAGTCCTTATCTGG46130.11338765846742556No Hit
TCCTCATCTTTCTGGA45840.11267483772267047No Hit
AATTCTGCTTAGCGAG45830.11265025769698923No Hit
CCCACCTCTTGACCTA45790.11255193759426439No Hit
TCCGTAAGATTACGCA45290.11132293631020386No Hit
TGTCTTCCTGAAATCA45160.11100339597634812No Hit
GAAGGACACCCAGCTG43940.1080046328432404No Hit
TCGTAGAACCGCACTG43240.10628403104555564No Hit
ATGGCCGTGGGCCAAA42880.10539915012103204No Hit
CCTGAGGACTGACTCA42660.1048583895560454No Hit
AGGTGATCTGTTAGTC41530.10208084665406858No Hit
TATGGGTACCCTTTGC41240.10136802590931346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)