FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18429094
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237410.1288234787884852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC29200.08.628563
TAGACTG25100.08.5324455
TACACTG33350.08.4363785
TATACTG23850.08.1579715
GTCTTAG21850.08.0094751
GCACCGT15750.07.8212826
CTACACT29400.07.80816134
TAGACAG32500.07.7525565
GTGTAGA28000.07.75031041
TACACAG39450.07.73508835
GTCTAGA22550.07.5745831
TGCACCG17400.07.561965
ATACCGT4852.0008883E-117.5042536
CCTAGAC25250.07.48378133
TATACAG25500.07.4654245
GTTCTAG25300.07.41531661
TACACCG8500.07.4105325
GTGTATA33300.07.3996961
GTCCTAG28650.07.3301371
GTATCGT2901.2915663E-57.24050956
Produced by FastQC (version 0.11.5)