FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004948259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839286
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA81191396.73853728049795No Hit
GGCAGGGA60230.717633798252324No Hit
GGCAAGGC30390.36209349375540634No Hit
GGCAAGAA26120.31121691533041185No Hit
GGCAACGA20750.2472339583884397No Hit
GGCAAGCA16890.20124248468340947Clontech Universal Primer Mix Long (100% over 8bp)
CGCAAGGA13880.1653786671051346No Hit
GCCAAGGA13670.16287654029734797RNA PCR Primer, Index 1 (100% over 8bp)
GGAAAGGA11520.13725952774143738No Hit
GGCAATGA9180.1093786861689579No Hit
GTCAAGGA8660.1031829435972958No Hit
GGCCAGGA8430.10044251899829142No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)