FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053452
Sequences flagged as poor quality0
Sequence length34
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCTT17240.16365244928103037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC2500.022.40292422
ATCTCCG2850.021.12155310
CTTATAC3050.019.744971
TTATACA3450.018.6646752
GAACCGT551.626041E-417.8171236
CCACGAG3500.017.20061120
TCTCCGA3600.017.11009611
CACGAGA3300.016.54604521
CCCACGA3700.016.27085119
TATACAC4050.016.2451783
ACATCTC4100.016.0478298
TACACAT4200.015.999055
TCCGAGC4050.015.89953713
TGAACCG550.00333077615.271825
TAGATAG550.00333077615.271825
ATCGGGC550.00333077615.271828
CGAGACC3950.014.17906723
GTTGTAT801.7480401E-414.0051521
ATACACA5050.013.8599064
CACATCT4650.013.848647
Produced by FastQC (version 0.11.5)