FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497257
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA343096998.10457166859628No Hit
GCCAAAAA98290.2810488334143016No Hit
CCCAACAA70270.20092889942031714RNA PCR Primer, Index 36 (100% over 8bp)
GACAACAA48880.13976668000092646No Hit
GCAAACAA43420.12415444446890807No Hit
GCCCACAA43360.12398288144108369No Hit
ACCAACAA42640.12192412510719115No Hit
GCCAACCA38070.1088567411545677No Hit
GCCACCAA35250.10079327884682196No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)