FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733984
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT669521.7930446407911762No Hit
GAAGCAGTGGAACCTT218540.5852729952779658No Hit
GTTCTTCCTAACGTAA188830.5057065054376237No Hit
CGCTTAAGATAATAGG160760.43053210726130586No Hit
CATGGAGGATCGACAT157790.4225781363819449No Hit
TGTCCCATGAGACAAT152770.40913405092255345No Hit
AAAGCATGATCTTTGT142700.3821655368635752No Hit
TGAGCGCACAGGACAC137210.36746274220778663No Hit
TGTCGAAACTTCGCAA128040.34290452235467533No Hit
TAATTGCCTGTCTGGC127570.34164581315827813No Hit
GATACGTACCGCTCGA120870.3237025118479351No Hit
GGGGGGGGGGGGGGGG110970.29718927558339836No Hit
GGAAGGACTAGGTAAT106500.285218147694259No Hit
AATGATGGAAAGCTAA103860.27814795135704923No Hit
CATGTCAGAGGTTTGT100980.27043500989827485No Hit
TCGACCTTGTTTCGCC94660.2535093883637423No Hit
ATGGGTACTTGGCTGC80080.21446262222869727No Hit
GTGAGATCTCGATTAC79530.21298966465844524No Hit
AAACGACCTAGGGAGG74320.19903673931114862No Hit
AGCCTACCTTAACTCG72480.19410902671248725No Hit
TGTTCCTCTTTATGGC71770.19220757239452552No Hit
TTGCGATGACATTCAG70990.19011865074944082No Hit
CAGCTCACTGTTTACG67910.18187008835602939No Hit
GCTATGATGACCTGCG67300.1802364445053862No Hit
CAATGTGACCAACGCC66860.1790580784491846No Hit
CTGCCTTGACCATAAC66370.17774580715932364No Hit
CCTGTTGACCCTAGTA65110.1743713952711099No Hit
TAACCAGACATCACAC63670.17051492454172273No Hit
CATGATGTGTCATGTG62700.16791716300873277No Hit
ATGCCGCGATGCCACG62390.16708695056004524No Hit
CACAGCTCTGGACTGA61290.16414103541954117No Hit
CATGCAAACGAAGGCT61240.1640071301858819No Hit
ATGGGTACTTTCGGGC60390.1617307412136742No Hit
TGGGCCATGCGTTCTC59380.1590258554937568No Hit
CTATTCAACCGAACGA57910.15508904162417408No Hit
TTGTTTGTGATACGCT57020.15270552846503893No Hit
AGAACGCGAGGAATAT56690.15182175392288774No Hit
ATTAGCGTGGTCGGTC56310.15080407414707722No Hit
CATTAGTCTATTCTGG56170.15042913949283127No Hit
CCGCTATTGTTCGCAA55100.14756356749252272No Hit
CACTATACTACGCCAC53490.14325181896869402No Hit
AGCTTGTGACTCACTC52740.1412432404638049No Hit
AACCAACACCGGAATG52120.13958281556642985No Hit
CCCAATGCTGGAATGC51310.13741355078114956No Hit
AACGTCCCTTACAGAA51100.1368511487997806No Hit
ATAGGAAACAGACTTC50870.13623518472494792No Hit
CTGCCATTGCACAGTT50840.13615484158475238No Hit
GGGCTGACTAATACAG50220.13449441668737736No Hit
TTGACGTGACCTCAAA49890.1336106421452261No Hit
AGCTCGGACCTTAAGC49400.13229837085536522No Hit
TTGTAACCTCATCCTC49280.13197699829458295No Hit
GGTAGCCTGTGCCACG49160.13165562573380069No Hit
CTGATTACTGCTTTGA49140.13160206364033697No Hit
ATAGCCAACATGGCCC49140.13160206364033697No Hit
TGGAGTTACAAGTCTC48700.13042369758413533No Hit
CGCTTACTGGCCACGT47730.12782593605114537No Hit
CGCTGAGTGTTAGCTG47690.12771881186421793No Hit
CCTACGGGATGGAACG47490.12718319092958086No Hit
GCAGATACTCGGCTCA47490.12718319092958086No Hit
TCAGGCTCTTAAAGCA46750.12520139347142353No Hit
CGATCAAACCGATCGC46640.12490680195737314No Hit
TGTTTGTTGCGCTCGA46420.12431761892927233No Hit
CGCTTCCCTGCGCTGT45810.12268397507862916No Hit
ATCTTACTGCGCACTG45790.12263041298516544No Hit
TCCAAAGTGAAGGCCA45690.12236260251784689No Hit
GCATCTCTGAGCCTAT45290.12129136064857268No Hit
GGGATCAGATGAGTAA44750.11984518412505249No Hit
TGGGTGCACTTCTGAG44550.11930956319041539No Hit
CATGTTACTAACGTGG44530.11925600109695168No Hit
AGCCGGTACGCGCAAA44420.11896140958290126No Hit
TACAACCGATTCCATT43670.11695283107801212No Hit
TTCTAACACTACGTTG43600.11676536375088913No Hit
CTCGGATCTTTCTGAG43320.11601549444239719No Hit
TATCTCCCTGGAATTA42740.11446219373194956No Hit
CCTTCAGTGTAGCTTG42680.11430150745155845No Hit
CGAGTCTCTTACTTTC42490.1137926675636532No Hit
ACCTGCTTGCCAAACT42020.11253395836725599No Hit
AGTGGTACTCAACGCC41870.11213224266627816No Hit
AACAGCTACTCGAGAG41820.11199833743261889No Hit
ATTGAGAGATGATCAC41710.11170374591856846No Hit
ATCGCAATGTAACTCG41550.11127524917085879No Hit
CCTCCCTTGTTTCGCC41230.11041825567543943No Hit
GATACCATGTCTGGAT41220.11039147462870756No Hit
TACGCCTTGGGTTGAG41130.11015044520812087No Hit
CTTAGGCCTTATGACC40860.10942735694636077No Hit
TTCACGCTGTGGGCAA40630.1088113928715281No Hit
CATGTCAGAACAGGTG40250.1077937130957176No Hit
GTGTACTGAATCACAC40210.10768658890879018No Hit
AGAACAATGAGTCGGA40170.10757946472186276No Hit
AACCACTGAAAACATG39830.10666890913297968No Hit
TGGTCCTTGTTTCTGT39700.10632075552546556No Hit
TGAGATAGACTCATCC39390.10549054307677805No Hit
ACCTCCATGGAATTGA39350.10538341888985062No Hit
CCTTCTTTGGATCATC39150.10484779795521353No Hit
GTTGCTAGACGCTCGA38830.10399080445979414No Hit
TCCGGGATGCGAGTTT38830.10399080445979414No Hit
GTCCTGACTTTACGAC38820.1039640234130623No Hit
GCATGGGCTATGTGGG38700.10364265085228003No Hit
TTCTATGACCAACGCC38480.10305346782417922No Hit
AGAACATGAAAGCTAA38480.10305346782417922No Hit
AGGTTACTGGTCCCGA38140.10214291223529615No Hit
AAAGGATACTGCTGGC37980.10171441548758645No Hit
CCATGATGAACAGGCA37840.10133948083334048No Hit
AGAGAATTGCCCGCAA37680.1009109840856308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)