FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5155059
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT500468197.0829043857694No Hit
CTAGCTGA296990.5761136778453942No Hit
CTAGCAGT105220.20411017604260204No Hit
CTCGCTGT92590.17960997148626234No Hit
CTAGATGT91330.17716577055665123No Hit
CTAGCTAT88510.17169541609514072RNA PCR Primer, Index 38 (100% over 8bp)
CAAGCTGT86780.16833948942194454No Hit
CTAGCTCT75140.1457597284531564No Hit
CTAGCTGG74380.14428544852735925No Hit
CTTGCTGT72180.140017796110578No Hit
CTACCTGT71210.13813614936317897No Hit
ATAGCTGT69690.13518758951158463No Hit
CTAGCGGT67060.1300858050315234No Hit
CTAGCTTT57410.11136632965791468No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)