FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15436573
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163030.10561281963295868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA34650.010.1672061
GTATAAG28700.08.830153
TGTATAA33250.08.63913252
TAGACTG20050.08.4469575
GTCTACG6150.08.4441851
GTCCTAG21000.08.4213631
CTAGACT20500.08.2623394
TAGACAG28000.08.1481475
TGGACTG26950.08.05011755
GTACTAG10450.07.79009631
TATAAGA34400.07.7722444
TCTAGAC17500.07.7607753
GTCCTAC12750.07.7058231
GTCGTAC2407.000785E-57.6026191
GTATAGA13550.07.5616181
TACACCG6700.07.52067765
CTTAGAC14900.07.51752953
CTAGGAC23850.07.51437763
AGTACCG5700.07.3667465
GTCTAGT15450.07.3584731
Produced by FastQC (version 0.11.5)