FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086834
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT537211.7403268202954871No Hit
GAAGCAGTGGAACCTT174910.5666323488726638No Hit
GTTCTTCCTAACGTAA166560.5395819794650442No Hit
CATGGAGGATCGACAT127670.41359528889470576No Hit
CGCTTAAGATAATAGG127050.41158675847162496No Hit
TGTCCCATGAGACAAT125300.40591751937421966No Hit
AAAGCATGATCTTTGT116890.378672776054689No Hit
TGAGCGCACAGGACAC111540.3613411022426214No Hit
TAATTGCCTGTCTGGC105470.3416769414876213No Hit
TGTCGAAACTTCGCAA102540.33218501545596557No Hit
GGAAGGACTAGGTAAT93770.3037740286649687No Hit
GATACGTACCGCTCGA85010.275395437525957No Hit
AATGATGGAAAGCTAA83660.27102202450795865No Hit
CATGTCAGAGGTTTGT82160.2661626767101827No Hit
GGGGGGGGGGGGGGGG78490.2542734724316241No Hit
TCGACCTTGTTTCGCC76910.24915495941796678No Hit
GTGAGATCTCGATTAC71040.2301387117026701No Hit
GCTATGATGACCTGCG64540.20908153791230755No Hit
ATGGGTACTTGGCTGC63370.20529124663004228No Hit
AAACGACCTAGGGAGG60360.19554015538250516No Hit
CAGCTCACTGTTTACG59810.19375839452332064No Hit
TGTTCCTCTTTATGGC59260.19197663366413614No Hit
AGCCTACCTTAACTCG59220.19184705105619546No Hit
TTGCGATGACATTCAG57240.18543271196313116No Hit
CAATGTGACCAACGCC51730.16758270771930073No Hit
CTGCCTTGACCATAAC51170.16576855120813105No Hit
TAACCAGACATCACAC50710.16427835121681308No Hit
CACAGCTCTGGACTGA50620.16398679034894653No Hit
AGCTTGTGACTCACTC50530.16369522948107995No Hit
CATGCAAACGAAGGCT49780.161265555582192No Hit
CCTGTTGACCCTAGTA49590.1606500381944737No Hit
CATGATGTGTCATGTG49580.1606176425424885No Hit
CTATTCAACCGAACGA48640.15757245125588223No Hit
ATGGGTACTTTCGGGC48380.15673016430426773No Hit
ATGCCGCGATGCCACG48030.15559631648478667No Hit
CATTAGTCTATTCTGG46840.15174123389855107No Hit
TTGTTTGTGATACGCT46670.15119050781480312No Hit
ATTAGCGTGGTCGGTC45930.1487932295679003No Hit
CACTATACTACGCCAC45680.14798333826827098No Hit
AGAACGCGAGGAATAT45020.14584522523724955No Hit
GCAGATACTCGGCTCA44130.14296201221056914No Hit
CCGCTATTGTTCGCAA42570.13790829050088213No Hit
CCCAATGCTGGAATGC42400.13735756441713418No Hit
GCATCTCTGAGCCTAT42150.13654767311750488No Hit
ATAGGAAACAGACTTC42130.13648288181353452No Hit
AGCTCGGACCTTAAGC41470.13434476878251309No Hit
GGGTCGTACCGCTCGA41230.13356727313486894No Hit
AACGTCCCTTACAGAA41190.13343769052692825No Hit
CTGCCATTGCACAGTT40540.131331973147892No Hit
TTGACGTGACCTCAAA40490.13116999488796613No Hit
AACCAACACCGGAATG40390.1308460383681144No Hit
GGGATCAGATGAGTAA40120.1299713557645147No Hit
TGGGCCATGCGTTCTC40100.12990656446054435No Hit
CTGATTACTGCTTTGA40050.1297445862006185No Hit
TTGTAACCTCATCCTC39990.12955021228870747No Hit
TGGAGTTACAAGTCTC39900.1292586514208409No Hit
TCAGGCTCTTAAAGCA39650.12844876012121156No Hit
ATAGCCAACATGGCCC38720.12543596448659047No Hit
GATACCATGTCTGGAT38370.1243021166671094No Hit
CCTACGGGATGGAACG38290.12404295145122801No Hit
CGCTGAGTGTTAGCTG37880.12271472971983594No Hit
CGATCAAACCGATCGC37750.12229358624402868No Hit
TGTTTGTTGCGCTCGA37720.12219639928807316No Hit
ATCTTACTGCGCACTG37410.12119213407653277No Hit
CGCTTACTGGCCACGT36990.1198315166931555No Hit
TTCTAACACTACGTTG36590.11853569061374858No Hit
GCGTCGATGCTTGAAC36570.11847089930977825No Hit
CATGTTACTAACGTGG36070.11685111671051959No Hit
TCCAAAGTGAAGGCCA36040.11675392975456407No Hit
TGGGTGCACTTCTGAG35830.11607362106287543No Hit
CTCGGATCTTTCTGAG35330.11445383846361676No Hit
CGCTTCCCTGCGCTGT35260.11422706889972056No Hit
GTAAGAAACGACAAAT34620.11215374717266947No Hit
GTGTACTGAATCACAC34290.11108469065715876No Hit
GCATAGTACGCTCCTT34230.11089031674524773No Hit
AACCACTGAAAACATG34110.11050156892142564No Hit
CGAGTCTCTTACTTTC34070.11037198631348497No Hit
GCATATCGAGCCGCTA33830.1095944906658408No Hit
TACGCCTTGGGTTGAG33660.10904376458209285No Hit
CCTTCAGTGTAGCTTG33640.1089789732781225No Hit
TGAGATAGACTCATCC33580.10878459936621146No Hit
CCTCCCTTGTTTCGCC33580.10878459936621146No Hit
TACAACCGATTCCATT33560.10871980806224113No Hit
GCGACAAGAAAGGCTG33520.10859022545430042No Hit
AACAGCTACTCGAGAG33260.10774793850268592No Hit
TTCACGCTGTGGGCAA33210.10758596024276006No Hit
ATTGAGAGATGATCAC33140.10735919067886383No Hit
CTTAGGCCTTATGACC33080.10716481676695282No Hit
TATCTCCCTGGAATTA33050.1070676298109973No Hit
AGTGGTACTCAACGCC33010.1069380472030566No Hit
GGTCAGAGAACCAGTT32860.106452112423279No Hit
TGGTCCTTGTTTCTGT32790.10622534285938279No Hit
AGAACAATGAGTCGGA32430.10505909938791655No Hit
ATCGCAATGTAACTCG32300.1046379559121093No Hit
GGTAGCCTGTGCCACG31980.10360129504858376No Hit
GGGCTGACTAATACAG31810.1030505689648358No Hit
TTCTATGACCAACGCC31730.10279140374895443No Hit
ATTCCTAACATTGCCA31720.10275900809696926No Hit
ACCTCCATGGAATTGA31620.10243505157711752No Hit
GAAAGTAACCCTGCGA31550.10220828201322131No Hit
AGCCGGTACGCGCAAA31460.10191672114535474No Hit
CATGTCAGAACAGGTG31040.10055610376197748No Hit
CCTTCTTTGGATCATC30930.10019975159014056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)