FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3706542
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC362004097.66623445788555Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA190070.5127960238950483No Hit
ATATGATC104950.283148012352214No Hit
ATCAGATC102190.27570171874485705Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGCTC79360.21410792053617628No Hit
AACTGATC57740.15577862061188028No Hit
ATCTGAAC56330.1519745358342088No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)