FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18429595
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC19250.011.85418222
CTTATAC25250.010.5393071
CCCACGA23050.010.44636519
ATCTCCG22450.010.35174510
CACGAGA22900.010.14813421
TATACAC39250.09.5237143
TCTCCGA24600.09.333199511
CTAGTAC11050.09.12231353
GACTCTA27350.08.75309826
TAGACAG33200.08.7291465
TACACTG36000.08.5557815
CCACGAG27900.08.37955820
TCCGAGC30550.08.29423213
ACTCTAT28750.08.27816527
TATACAG26600.08.2633765
GTATTAG20700.08.2548521
TACACAT46100.08.1390435
TATACTG22700.08.0178335
TTATACA36000.07.97236732
TAGACCG6200.07.9034345
Produced by FastQC (version 0.11.5)