FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18720524
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC18150.012.41792622
CGAGACA20900.010.85099923
CCCACGA23300.09.85330519
CACGAGA23950.09.64431321
CTTATAC26000.09.5881411
TATACAC40650.09.5075843
TCTCCGA25100.09.31411111
ATCTCCG24800.09.25753810
TATACTG22700.08.9448025
TAGACAG33950.08.909285
TTATACA34800.08.450122
TACACTG32300.08.4106165
TAGACCG7550.08.3463045
CTTAGAC19750.08.2245583
CCACGAG28350.08.14746720
TACACAT44750.08.0420865
GTCCTAG28900.07.8506381
TAGACTG26300.07.8269075
TCTAGAC20200.07.76404953
CTAGACT25700.07.68267154
Produced by FastQC (version 0.11.5)