FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186557
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT582001.8264226875590175No Hit
GAAGCAGTGGAACCTT196880.6178455304581089No Hit
GTTCTTCCTAACGTAA166690.5231037762701247No Hit
CATGGAGGATCGACAT134130.4209245276328024No Hit
CGCTTAAGATAATAGG134020.42057932746848714No Hit
TGTCCCATGAGACAAT132870.4169704166597365No Hit
AAAGCATGATCTTTGT123260.38681247503182903No Hit
GATACGTACCGCTCGA120310.37755483426155567No Hit
TGAGCGCACAGGACAC118590.37215715896498947No Hit
TAATTGCCTGTCTGGC108990.34203059917020157No Hit
TGTCGAAACTTCGCAA107920.33867274302640754No Hit
GGGGGGGGGGGGGGGG93270.29269835750623635No Hit
GGAAGGACTAGGTAAT92660.29078406568594256No Hit
AATGATGGAAAGCTAA87770.27543834929047245No Hit
CATGTCAGAGGTTTGT86270.27073107432253685No Hit
TCGACCTTGTTTCGCC82240.2580841955753498No Hit
GTGAGATCTCGATTAC68400.21465173853786393No Hit
ATGGGTACTTGGCTGC67540.21195290088958083No Hit
AAACGACCTAGGGAGG64110.20118893212956804No Hit
AGCCTACCTTAACTCG60950.1912722728637837No Hit
GCTATGATGACCTGCG60230.1890127808791746No Hit
CAGCTCACTGTTTACG59380.1863453250640111No Hit
TGTTCCTCTTTATGGC59200.18578045206785881No Hit
TTGCGATGACATTCAG59030.18524696090482612No Hit
CTGCCTTGACCATAAC56190.17633452029886804No Hit
CCTGTTGACCCTAGTA55610.1745143739779329No Hit
CAATGTGACCAACGCC54550.17118789966725842No Hit
CACAGCTCTGGACTGA54020.16952466251192116No Hit
TAACCAGACATCACAC53840.1689597895157689No Hit
CATGATGTGTCATGTG52900.16600989720252926No Hit
CATGCAAACGAAGGCT52000.16318553222176788No Hit
ATGCCGCGATGCCACG51700.16224407722818077No Hit
ATGGGTACTTTCGGGC51270.16089465840403921No Hit
CTATTCAACCGAACGA50160.15741127492776688No Hit
TTGTTTGTGATACGCT50010.1569405474309733No Hit
TGGGCCATGCGTTCTC49350.15486934644508163No Hit
CATTAGTCTATTCTGG48530.15229603612927683No Hit
AGCTTGTGACTCACTC48410.15191945413184196No Hit
AGAACGCGAGGAATAT48290.15154287213440715No Hit
ATTAGCGTGGTCGGTC48040.15075832630641786No Hit
CCGCTATTGTTCGCAA47740.14981687131283075No Hit
CACTATACTACGCCAC46960.14736908832950424No Hit
GGGCTGACTAATACAG44670.14018264854512252No Hit
AACCAACACCGGAATG44250.13886461155410054No Hit
ATAGGAAACAGACTTC43730.13723275623188286No Hit
CCCAATGCTGGAATGC43640.13695031973380672No Hit
AGCTCGGACCTTAAGC43430.13629130123829575No Hit
GCAGATACTCGGCTCA43080.1351929370791108No Hit
AACGTCCCTTACAGAA42960.13481635508167591No Hit
TTGACGTGACCTCAAA42780.13425148208552365No Hit
GGTAGCCTGTGCCACG42610.13371799092249095No Hit
CTGCCATTGCACAGTT41660.13073671677613172No Hit
TGGAGTTACAAGTCTC41480.13017184377997945No Hit
CTGATTACTGCTTTGA41270.12951282528446847No Hit
ATAGCCAACATGGCCC41240.12941867978510976No Hit
TCAGGCTCTTAAAGCA41160.12916762512015317No Hit
GCATCTCTGAGCCTAT40970.12857137029088136No Hit
TTGTAACCTCATCCTC40380.12671984213682666No Hit
TGTTTGTTGCGCTCGA40290.12643740563875053No Hit
CCTACGGGATGGAACG39700.12458587748469586No Hit
CGCTGAGTGTTAGCTG39680.12452311381845671No Hit
TCCAAAGTGAAGGCCA39460.12383271348982616No Hit
TGGGTGCACTTCTGAG39260.12320507682743476No Hit
GATACCATGTCTGGAT39240.1231423131611956No Hit
GGGATCAGATGAGTAA39190.12298540399559776No Hit
ATCTTACTGCGCACTG38910.12210671266824978No Hit
CGCTTACTGGCCACGT38840.12188703983641279No Hit
TTCTAACACTACGTTG38610.12116525767466264No Hit
CGATCAAACCGATCGC38250.12003551168235811No Hit
CGCTTCCCTGCGCTGT37590.11796431069646643No Hit
CATGTTACTAACGTGG37450.11752496503279244No Hit
CTCGGATCTTTCTGAG37360.11724252853471631No Hit
AGCCGGTACGCGCAAA36710.11520270938194421No Hit
TACGCCTTGGGTTGAG36420.11429263622147667No Hit
CTTAGGCCTTATGACC36170.11350809039348739No Hit
CGAGTCTCTTACTTTC36090.11325703572853081No Hit
ACCTGCTTGCCAAACT35880.11259801723301985No Hit
TACAACCGATTCCATT35880.11259801723301985No Hit
CCTCCCTTGTTTCGCC35640.11184485323815015No Hit
CCTTCAGTGTAGCTTG35500.11140550757447615No Hit
AGTGGTACTCAACGCC35010.10986779775161719No Hit
TATCTCCCTGGAATTA34950.10967950675289977No Hit
TCCGGGATGCGAGTTT34910.10955397942042147No Hit
AGAACAATGAGTCGGA34820.10927154292234534No Hit
ATTGAGAGATGATCAC34800.1092087792561062No Hit
TTCACGCTGTGGGCAA34510.10829870609563866No Hit
GCATGGGCTATGTGGG34470.10817317876316036No Hit
ATCGCAATGTAACTCG34430.10804765143068208No Hit
GTGTACTGAATCACAC34310.10767106943324724No Hit
AACAGCTACTCGAGAG34280.10757692393388851No Hit
AACCACTGAAAACATG34130.10710619643709496No Hit
ACCTCCATGGAATTGA34030.10679237810589925No Hit
CATGTCAGAACAGGTG33900.10638441427534484No Hit
TGAGATAGACTCATCC33710.10578815944607299No Hit
GTCCTGACTTTACGAC33620.10550572294799686No Hit
GTTGCTAGACGCTCGA33270.10440735878881188No Hit
TGGTCCTTGTTTCTGT33220.104250449623214No Hit
AAAGGATACTGCTGGC33040.10368557662706174No Hit
AGAACATGAAAGCTAA32720.10268135796723549No Hit
AGGTTACTGGTCCCGA32590.10227339413668107No Hit
GTAAGAAACGACAAAT32550.10214786680420279No Hit
TTCTATGACCAACGCC32490.10195957580548536No Hit
AGAGAATTGCCCGCAA32460.10186543030612666No Hit
TTCGACCACAGTACGG32440.1018026666398875No Hit
CGGTTCACTGGAATGC32300.1013633209762135No Hit
ATTCCTAACATTGCCA31970.10032772048326767No Hit
GCATATCGAGCCGCTA31920.10017081131766983No Hit
CCTTCTTTGGATCATC31910.10013942948455025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)