FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004948464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827667
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC51420.6212643490679223No Hit
CGACCAGACGCGCTGT44170.5336687339231841No Hit
CTCCTAGTGAACGTAA43450.5249695831777756No Hit
TAGCTTTACCCTATCC39460.47676178946363695No Hit
CCAGGACTGTAACTCG29670.3584775036337078No Hit
ATCTGTACTGGGACTA27330.3302052637111302No Hit
GTTCTCGACCGTAGAG26470.3198146114318923No Hit
CTTCCTTTGGCTCAGC26240.3170357160548868No Hit
AGACCATTGAATCCAC25540.3085782083857397No Hit
CGATCAAACCCGGCTA24930.3012080945597686No Hit
TGCTGCTACTTTCTTG21910.26471999004430524No Hit
ACAGCCGACTTGCGTA20720.2503422270067551No Hit
GGGGGGGGGGGGGGGG18900.22835270706697258No Hit
AACTTACCTCACCTAA18490.22339902400361497No Hit
GGCAACAGAATAGCAA18450.2229157378510923No Hit
CGCGTTGGAGCGGTTC18060.21820369786399604No Hit
ATCGAAAGATTTCCGC17310.20914208250419555No Hit
CCCTGAACTAGCTTTG16700.20177196867822447No Hit
CAATTCGGATAACGGG16190.1956100702335601No Hit
GTAACTGTGTGAAAGT15740.1901731010176798No Hit
AGTGGAGTGAGTTGAA15210.18376955949675414No Hit
GGGTAGAACCAGTCTA14890.1799032702765726No Hit
AAGAACCCTTAACCCA14740.1780909472046125No Hit
TCCGGGAACGTTCTCC13580.16407564878145436No Hit
TTCCTAAACTAATGCC13550.16371318416706235No Hit
TGCAGATACTGCCACG13380.16165921801884092No Hit
TGGAACACTCCTAGTA13330.16105511032818753No Hit
CGCGATCGATATCTGG13250.16008853802314216No Hit
TTTAGTCTGAGCAATA13070.15791375033679003No Hit
GTGAAGCGATCCCAGG12970.1567055349554833No Hit
CTTTAACTGTCCGTAA12570.15187267343025637No Hit
AGTCCTGCTCGAACGA12420.15006035035829626No Hit
TGATAGAGAGACTGTT12280.14836884882446683No Hit
TGCAGTATGACACCGC12010.14510666729493868No Hit
CACATCCTGAGCCTAT11760.14208612884167182No Hit
TCTCAGAGATGCATGT11590.14003216269345037No Hit
AGGTGTGGATCATGTG11580.13991134115531972No Hit
GCAACTTGAAGGTGCA11450.13834066115962096No Hit
GAGTGTTACACCTGCG11330.1368908027020529No Hit
CTTAGATCTTGTCACA11330.1368908027020529No Hit
CATAGGTACAAGGTGC11280.1362866950113995No Hit
TCAAAGCACTCTGCAA11220.13556176578261547No Hit
ATTTCTGACGGGTAGT11150.13471601501570074No Hit
CTATTGGGACTTGTGA11150.13471601501570074No Hit
TGAACTGTGAACGTAA11060.1336286211725247No Hit
TTCTTAGACGTCCCGA10890.13157465502430327No Hit
TACGTTTACTGCACCG10890.13157465502430327No Hit
CGAGTCTCTGCTACGC10800.1304872611811272No Hit
TCTATGGGATGCTCAA10740.12976233195234316No Hit
AGAGTACCTTAATCGC10680.12903740272355912No Hit
TGAGGGATGTACGTTG10670.12891658118542845No Hit
ATGTACGTGTACTTTC10520.12710425811346834No Hit
TGGCAATTGAGTGGTG10480.12662097196094563No Hit
TCCGGGTGAACACCTA10110.12215057505011073No Hit
GGTCGCATGGTACTAC10050.1214256458213267No Hit
GGTCTAAACTCCTACC9860.11913003659684392No Hit
TCCATTTGAAATCTGC9830.11876757198245189No Hit
GGGTGTGCTCGCAAAG9820.11864675044432121No Hit
CTAGCTCACGAATAGT9770.11804264275366784No Hit
CACCAGGTGCGCCACA9690.11707607044862246No Hit
CTACAACGAATCTGTG9640.11647196275796909No Hit
GGGATGTGACTGGAAG9490.114659639686009No Hit
GTAATCTCTACACAAA9420.11381388891909427No Hit
TGCTAGTGAAGGCGTA9370.11320978122844091No Hit
TACTGTTTGCTTAAGC9370.11320978122844091No Hit
TTGCGGTCTTTTGGGT9330.11272649507591821No Hit
CTTTCGTCTCGAACGA9260.11188074430900351No Hit
AGACTACTGGCCCTAG9250.11175992277087284No Hit
CGAGTCTCTTGGAACG9250.11175992277087284No Hit
AGCCAATTGTTATGGC9150.1105517073895661No Hit
ACCAAGTTGAAGCTAA9140.11043088585143543No Hit
ACCTCAGCTAATCCGT9130.11031006431330476No Hit
TGTAAGCACGCGCTGT9090.10982677816078205No Hit
CTCAATTTGGAATCTC9070.10958513508452072No Hit
TCGGACCGATAGGCGA9070.10958513508452072No Hit
CATAGTCGAAGATTCC9050.10934349200825935No Hit
GGCTGACTGAAACTGA9010.10886020585573666No Hit
TGCGCAGACATCAGGG8860.10704788278377657No Hit
GTGTGATACCGTGCAC8860.10704788278377657No Hit
CATTCTATGCCTAGCG8820.10656459663125387No Hit
GCATGATACTTGCGTA8810.10644377509312321No Hit
ATGTCCAGACCGCTAG8720.10535638124994713No Hit
CTAACAGTGCATGATC8620.10414816586864041No Hit
GCCATTCACCGGAAGT8550.10330241510172569No Hit
ATAGCCAACCGTTCTC8530.10306077202546435No Hit
AGGCGATGACCTGCGA8430.10185255664415761No Hit
GGGCCTTGACTATCTT8400.1014900920297656No Hit
GTATTCACTGCTCCTT8390.10136927049163494No Hit
ACGAGGATGAACTAGA8340.10076516280098156No Hit
TAGTACCGAACGAGCA8330.10064434126285088No Hit
CACATTACTGATGGAC8320.1005235197247202No Hit
GCACTGTCTCGATGGA8320.1005235197247202No Hit
CAAGGGAACTGATGAA8310.10040269818658953No Hit
TACCGCTTGATCCTAG8310.10040269818658953No Hit
TTTAGGGACCACAGTT8280.10004023357219752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)