FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801358
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT408801.0754051578409611No Hit
TGCTGCTACCGTTCTC368920.9704952808969847No Hit
AAACGACCTCTTCAAG364480.9588152444468528No Hit
ATGGGTTACAATCGAA359700.9462407907910805No Hit
TATGCTTTGGACTGTT355150.9342713840685355No Hit
GCCTCTTGATCTGTGA333920.8784229214927929No Hit
AAAGCGGACTCGGGCT322130.8474076895677809No Hit
TCCAAAGTGCTCACGG311820.820285803126146No Hit
GCAGGTCTGGCTGTTA295200.7765645856033555No Hit
TAGTGCATGAACCCGC293930.7732236742764034No Hit
GAAGTATGAACAGGTG284530.7484956691792775No Hit
TAACTCACTGGTGTCC280170.7370260838363553No Hit
TGTGGCTCTTACGATC271080.7131135767796667No Hit
TTCCCATCTCTTTGTT244710.6437436305657084No Hit
TCACCTGACTCAATGC236350.6217514898623071No Hit
TGAGAATGAGCAGTTT234530.6169637271732891No Hit
GAGCCTGACCGAGTTT230270.605757205714379No Hit
GGAACTACTACCAAGG229710.6042840479639118No Hit
CTAGAGACTAATCTGC218680.5752681015573908No Hit
ATGTCCAGAGTCGGTC218060.5736371054765165No Hit
GAAGTAATGAGTCTGT217480.572111334949247No Hit
TCGTATACTGCTAATC214690.5647718525853129No Hit
TCACACAGATTGGGAG213400.5613783284815584No Hit
TTCGTCCGACCTGTGG210490.5537231694568099No Hit
TGCGGTGACCTTAGTG210040.552539381978756No Hit
ATTCCTAACTGCTACA207560.546015397655259No Hit
AGGTCGAGAAGGGAGG204140.5370186128220494No Hit
TCCGCGAGAGAACAGC202670.5331515737270733No Hit
ACTGATTCTCGGCTCA199280.5242337080590673No Hit
TGGACCTCTAGGGAGG196960.5181306259499895No Hit
TGGCCTGGACCCGAGT192710.5069504108794805No Hit
CCATGATGATTCGGGC186090.4895355817578876No Hit
GCAATCATGGAACGTG176530.4643866744463426No Hit
TTCGATGGAAATCCTG173240.45573187266234855No Hit
ACGAGGATGGAGCTGA168830.44413075537742036No Hit
AAGGTGCGAACAGATA165610.4356600983122347No Hit
TCCCAGACTGTCTTGA161900.42590042821539037No Hit
TTTCGTAACAGTACGG161070.423716997978091No Hit
TGGTCCTTGGAGAGGG156700.41222110624676755No Hit
TCTCTCTACTCTGGAT153860.40475009194082745No Hit
GCGGTTCGAGTACGTT144040.3789172185308513No Hit
TCGCCGAGAAGGTAAT134970.35505732425096503No Hit
TTCGATGGACAGGTGA134350.3534263281700908No Hit
TCCTTCACTTGTCACA134070.3526897492948573No Hit
TGATATCCTCGATGTT131710.34648144163217465No Hit
AGCCCAGTGAAAGTAC130390.3430089983632165No Hit
AGCTGCTCTTGTCACA128100.3369848354193422No Hit
AACTCCCTGCTTCACC128070.3369059162541386No Hit
CTTTCGGTGCTTTGTT126410.33253905577953985No Hit
AACCCTCACGCGGTTC125540.33025039998863565No Hit
ATGGCCGTGATACGCT121290.3190701849181266No Hit
CCCTTGCCTCCTACGG120380.31667630357361765No Hit
TCGGCTTACCTCCTAC118970.31296710280904877No Hit
TGTTTGTTGCAAGTTC113440.2984196700231864No Hit
GGGGGGGGGGGGGGGG111980.2945789373166116No Hit
CTAACTGGAACCTTGA109900.28910720852916244No Hit
AAACACGCTGCAGTTT109330.2876077443902942No Hit
ACCCTTGTGGGCCATC107670.28324088391569535No Hit
TGGCCTTTGCGAAAGC106290.2796106023163301No Hit
AAATAGGACTCTATCT103800.2730603116044319No Hit
TACAACGTGAGCAGGT103400.27200805606838396No Hit
CAATGTGTGGTTGACT102250.2689828214022463No Hit
TCAAAGGCTCTTAGTG101390.26672047199974325No Hit
CTTTCGGTGAGCCATT97970.25772368716653365No Hit
TGAACTGTGTGCTGGC96510.2538829544599588No Hit
AGTGTTCGATTCGCCT90040.2368627211643839No Hit
CAGCCGATGTAATAGG89700.2359683039587432No Hit
GAGGTTGGAGGAATAT88050.23162774987254553No Hit
GGCTTGCGAATACGCT84660.22270988420453955No Hit
ACAGCCGACAAACATG82440.21686986597947364No Hit
GATAAGAACATTGGTC81450.21426553352775507No Hit
GTGCTAATGGGAGTCA79120.20813614503027603No Hit
TTGGGAGGAATCTGTG79080.2080309194766712No Hit
GTAAAGCCTGAAATCA76540.201349096822767No Hit
CGACGTTCTTGCTACA76290.2006914371127371No Hit
TGTACTGGAAATGCTC75240.19792926633061134No Hit
AGGGACCCTAATCCTG71570.1882748217873718No Hit
GGAACATCTTTACGAC70380.18514436156762926No Hit
TCTTGAACTCGTACAT69220.18209282051309034No Hit
TAACGAGCTTACTTTC69010.18154038635666517No Hit
GGACATTTGGCCGCTA68810.1810142585886412No Hit
GGGTGAAACCGACTAT68090.17912019862375497No Hit
TACCACCTGAAACATG67510.1775944280964855No Hit
CGGATGAGAACAGGCA67330.17712091310526396No Hit
TACGTGTGAAACGCAG66250.1742798231579346No Hit
GGACCACTGGCTAGAG65160.17141242682220406No Hit
CATGATGTGCCTAGTA64840.17057062239336573No Hit
AGCTTGTGAACCGTGC64840.17057062239336573No Hit
ACTTATCTGTCAGGTT64130.1687028688168807No Hit
AACTAGCACTGCCACG62270.16380988057425794No Hit
ACGTGTTGATCTTTGT59850.15744373458116812No Hit
TCGGACCGAATGTCCA59560.15668084931753337No Hit
TGGTAGGGATGTGCTA57840.15215615051252737No Hit
AACTACGACATTGCCA57470.15118281414168305No Hit
CGTCGTACTTGCGCCA55680.14647397061786868No Hit
CTCGGATCTAGTACGG55310.14550063424702436No Hit
GTAGATAGAGCTGAGA55170.14513234480940757No Hit
CCTTCAGTGCAACGCC54510.14339612317492853No Hit
GGTAGTTTGTCCAGCG53980.14200188458966506No Hit
AACGAACCTGACACCC53720.14131791849123393No Hit
TTTAGGGACTGGGCAA52990.1393975521379465No Hit
AGTTTGCGAGCAGGAT52570.13829268382509619No Hit
TACCACCTGTACTTTC51950.13666168774422194No Hit
TGAGGAGTGGTCCCAG51830.13634601108340755No Hit
GTCCGAGTGGTCTTGA51310.13497807888654528No Hit
CTTTAACTGTTCGCCT50340.13242635921162912No Hit
GCCATAATGACCGTTA49400.12995355870191655No Hit
CAGGCCATGTCGTAGT48480.12753337096900635No Hit
GTCCGTTACGGATGGT47730.1255603918389165No Hit
AGAACGCGACACAGTT47720.12553408545051534No Hit
CTTACATTGGAAGGCT47480.12490273212888657No Hit
GAGAAGAGATGCTACA46910.1234032679900183No Hit
GAAAGTCCTTATCTGG45870.12066740359629376No Hit
TGCAATGACTCCGTAA45850.12061479081949135No Hit
GGGATGTGATGCATGT44760.1177473944837608No Hit
AACTACGACGCTGTTA44050.11587964090727577No Hit
GAACCATACTTTGGGT43520.11448540232201229No Hit
CCGTTACTGTTGGTTG43460.11432756399160511No Hit
GCTGGACACATTGGTC43270.11382774261198235No Hit
GAAGGACACCCAGCTG42610.1120915209775033No Hit
CGATCGGTGCGAGGAT42590.1120389082007009No Hit
GGCTTAAACTGCATCA42530.11188106987029371No Hit
TCCTCATCTTTCTGGA41810.10998700990540748No Hit
AATTCTGCTTAGCGAG41590.10940826936058114No Hit
CAAAGACCTGCTTTGA41430.10898736714616197No Hit
ATGGCCGTGGGCCAAA40890.10756682217249731No Hit
TGTCTTCCTGAAATCA40870.1075142093956949No Hit
TCGTAGAACCGCACTG40820.10738267745368892No Hit
TCCGTAAGATTACGCA40300.10601474525682665No Hit
GCGTTTAGAAGTCTAC40250.10588321331482065No Hit
ATAGCCAACATCTGTG39780.10464681305996436No Hit
CCCACCTCTTGACCTA39760.10459420028316196No Hit
TATGGGTACCCTTTGC39650.10430483001074879No Hit
CCTGAGGACTGACTCA39340.10348933197031167No Hit
ACAAGACCTAAGCTAA39030.10267383392987453No Hit
AGGTGATCTGTTAGTC38220.10054301646937752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)