FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18429595
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262290.14232000214871787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG19750.08.7176945
GTCCTAG28050.08.5353951
TAGACTG23100.08.2412025
GTGTATA35200.08.2335671
CTAGGAC27750.08.171453
CCTACAC28400.08.1322873
TACACTG33950.07.91635135
TCTATAC18450.07.8901293
CTATACT21250.07.7069044
CTAGACT22550.07.6971184
TAGACAG27750.07.6168885
GTGTAGG29950.07.5264371
TAGTACG3352.2162021E-77.5210894
TAGTACT16250.07.49408964
AGGACCG19250.07.4897985
CTACACT32200.07.47696924
GGACTGT32250.07.4658636
CTAGTAC11700.07.41742133
ATACTGT30350.07.3797666
AGACTGT41450.07.3285246
Produced by FastQC (version 0.11.5)