FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058558
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT360941.7533632766237337No Hit
GAAGCAGTGGAACCTT124560.6050837528017184No Hit
GTTCTTCCTAACGTAA111820.5431957710202967No Hit
CGCTTAAGATAATAGG84790.41189026493302594No Hit
CATGGAGGATCGACAT83490.40557516475124816No Hit
TGTCCCATGAGACAAT80210.38964168121568593No Hit
AAAGCATGATCTTTGT78720.38240360485349456No Hit
GATACGTACCGCTCGA76490.37157077915706044No Hit
TGAGCGCACAGGACAC74220.36054364268580236No Hit
TGTCGAAACTTCGCAA70270.3413554536719393No Hit
TAATTGCCTGTCTGGC67640.3285795202272659No Hit
GGAAGGACTAGGTAAT63480.30837119964557713No Hit
CATGTCAGAGGTTTGT55730.2707234870234407No Hit
GGGGGGGGGGGGGGGG54250.26353398835495523No Hit
AATGATGGAAAGCTAA53780.2612508367507741No Hit
TCGACCTTGTTTCGCC52000.2526040072711092No Hit
GTGAGATCTCGATTAC46910.22787796117476408No Hit
GCTATGATGACCTGCG44580.21655935854127012No Hit
ATGGGTACTTGGCTGC42930.20854403907978303No Hit
AAACGACCTAGGGAGG41270.20048014192458993No Hit
CAGCTCACTGTTTACG39980.1942136194365182No Hit
TGTTCCTCTTTATGGC38260.18585825611908918No Hit
TTGCGATGACATTCAG38240.18576110073167723No Hit
AGCCTACCTTAACTCG37540.18236066217225844No Hit
AGCTTGTGACTCACTC35530.17259654573735594No Hit
CTGCCTTGACCATAAC34920.169633306421291No Hit
TAACCAGACATCACAC34640.16827313099752353No Hit
CAATGTGACCAACGCC34600.16807882022269957No Hit
CACAGCTCTGGACTGA33440.1624438077528056No Hit
ATGCCGCGATGCCACG33060.16059785539197827No Hit
CCTGTTGACCCTAGTA33000.16030638922974239No Hit
CATGATGTGTCATGTG32380.15729457221997145No Hit
CATGCAAACGAAGGCT31920.15505999830949627No Hit
ATGGGTACTTTCGGGC31540.15321404594866891No Hit
CTATTCAACCGAACGA31290.15199960360601936No Hit
CATTAGTCTATTCTGG31050.15083373895707577No Hit
ATTAGCGTGGTCGGTC30960.15039653971372194No Hit
TTGTTTGTGATACGCT30200.14670463499206726No Hit
AGAACGCGAGGAATAT29990.1456845034242416No Hit
GCATCTCTGAGCCTAT29680.14417859491935617No Hit
CACTATACTACGCCAC29650.1440328618382382No Hit
ATAGGAAACAGACTTC28410.13800922781869637No Hit
GCAGATACTCGGCTCA28400.1379606501249904No Hit
AGCTCGGACCTTAAGC28170.13684336316975282No Hit
GGGTCGTACCGCTCGA28050.136260430845281No Hit
CCCAATGCTGGAATGC27250.13237421534880242No Hit
GATACCATGTCTGGAT27240.13232563765509645No Hit
AACGTCCCTTACAGAA27200.13213132688027251No Hit
AACCAACACCGGAATG27120.13174270533062465No Hit
TTGTAACCTCATCCTC26880.13057684068168107No Hit
CCGCTATTGTTCGCAA26770.13004248605091526No Hit
GGGATCAGATGAGTAA26710.12975101988867935No Hit
TGGAGTTACAAGTCTC26690.1296538645012674No Hit
TTGACGTGACCTCAAA26500.12873088832085372No Hit
TGGGCCATGCGTTCTC26410.12829368907749988No Hit
CTGCCATTGCACAGTT25980.12620484824814263No Hit
TGTTTGTTGCGCTCGA25980.12620484824814263No Hit
CGCTTACTGGCCACGT25590.12431031819360931No Hit
CCTACGGGATGGAACG25570.12421316280619736No Hit
GCGTCGATGCTTGAAC25490.12382454125654949No Hit
TCAGGCTCTTAAAGCA25400.12338734201319565No Hit
CTGATTACTGCTTTGA24990.12139565657125036No Hit
ATAGCCAACATGGCCC24890.12090987963419055No Hit
CGCTGAGTGTTAGCTG24870.12081272424677857No Hit
TCCAAAGTGAAGGCCA24680.1198897480663649No Hit
CGATCAAACCGATCGC24500.11901534957965722No Hit
CATGTTACTAACGTGG24480.11891819419224525No Hit
ATCTTACTGCGCACTG24320.11814095109294953No Hit
CTCGGATCTTTCTGAG24270.11789806262441961No Hit
GTAAGAAACGACAAAT24230.11770375184959568No Hit
GGGCTGACTAATACAG24200.11755801876847773No Hit
TGGGTGCACTTCTGAG24080.11697508644400595No Hit
CGCTTCCCTGCGCTGT23790.11556633332653246No Hit
CGAGTCTCTTACTTTC23730.11527486716429655No Hit
TTCTAACACTACGTTG23710.1151777117768846No Hit
TACGCCTTGGGTTGAG23380.11357464788458718No Hit
GGTAGCCTGTGCCACG23010.1117772732174658No Hit
GCATAGTACGCTCCTT22880.11114576319928805No Hit
GCGACAAGAAAGGCTG22780.11065998626222821No Hit
CTTAGGCCTTATGACC22610.10983416546922652No Hit
CCTCCCTTGTTTCGCC22610.10983416546922652No Hit
ATTGAGAGATGATCAC22490.10925123314475473No Hit
AGTGGTACTCAACGCC22430.10895976698251883No Hit
CCTTCAGTGTAGCTTG22350.10857114543287097No Hit
GTGTACTGAATCACAC22200.10784248002728124No Hit
TACAACCGATTCCATT22180.10774532463986926No Hit
AACAGCTACTCGAGAG22030.10701665923427953No Hit
AACCACTGAAAACATG21840.10609368305386586No Hit
GAAAGTAACCCTGCGA21780.10580221689162995No Hit
TGAGATAGACTCATCC21660.10521928456715818No Hit
TTCTATGACCAACGCC21650.1051707068734522No Hit
AGAACAATGAGTCGGA21630.10507355148604022No Hit
CATGTCAGAACAGGTG21610.10497639609862827No Hit
GCATATCGAGCCGCTA21590.10487924071121629No Hit
GGTCAGAGAACCAGTT21450.10419915299933254No Hit
AGCCGGTACGCGCAAA21430.10410199761192059No Hit
ATCGCAATGTAACTCG21300.1034704875937428No Hit
TATCTCCCTGGAATTA21120.10259608910703512No Hit
ACCTCCATGGAATTGA21000.10201315678256334No Hit
TGGTCCTTGTTTCTGT20920.10162453523291547No Hit
GGCTTGCGAGTACGTT20910.10157595753920948No Hit
TTCACGCTGTGGGCAA20860.10133306907067959No Hit
AGAACATGAAAGCTAA20830.10118733598956163No Hit
ATTCCTAACATTGCCA20710.10060440366508984No Hit
GTTGCTAGACGCTCGA20640.10026435980914797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)