FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004948490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522666
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA539345597.66035099714522No Hit
CCCAACAA205110.3713967131092121RNA PCR Primer, Index 36 (100% over 8bp)
GCCAAAAA157270.28477188372427376No Hit
GACAACAA119210.21585589278801218No Hit
GCCCACAA100920.18273782988143772No Hit
GCAAACAA90040.1630371997872042No Hit
ACCAACAA85490.15479842525331064No Hit
GCCACCAA67830.12282111574373682No Hit
GCCAACAC63010.11409344689684293No Hit
GCCAACCA59520.10777403522139488No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)