FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145297
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC268950.5227103508310599No Hit
CTCCTAGTGAACGTAA268380.5216025430601966No Hit
CGACCAGACGCGCTGT253030.4917694741430864No Hit
TAGCTTTACCCTATCC235240.457194210557719No Hit
CCAGGACTGTAACTCG185300.3601347016508474No Hit
ATCTGTACTGGGACTA172380.3350243921779442No Hit
CTTCCTTTGGCTCAGC160730.3123823561594209No Hit
GTTCTCGACCGTAGAG155400.30202338174064586No Hit
CGATCAAACCCGGCTA148620.2888462998345868No Hit
AGACCATTGAATCCAC139460.2710436346045719No Hit
GGGGGGGGGGGGGGGG131630.2558258541732382No Hit
TGCTGCTACTTTCTTG131280.25514562133148No Hit
ACAGCCGACTTGCGTA123260.2395585716431918No Hit
AACTTACCTCACCTAA111620.21693597084871874No Hit
CGCGTTGGAGCGGTTC105820.20566354089958266No Hit
ATCGAAAGATTTCCGC104410.20292317430849957No Hit
CCCTGAACTAGCTTTG100220.1947798154314513No Hit
AGTGGAGTGAGTTGAA96870.1882690153746227No Hit
GTAACTGTGTGAAAGT96710.18795805178981895No Hit
CAATTCGGATAACGGG93530.18177765054184433No Hit
GGGTAGAACCAGTCTA85220.16562697935609938No Hit
TTCCTAAACTAATGCC85020.16523827487509465No Hit
AAGAACCCTTAACCCA84260.16376119784727686No Hit
TTTAGTCTGAGCAATA83670.162614519628313No Hit
CGCGATCGATATCTGG82150.15966036557267735No Hit
TCCGGGAACGTTCTCC78990.1535188347728032No Hit
GTGAAGCGATCCCAGG78780.15311069506774827No Hit
GGCAACAGAATAGCAA78780.15311069506774827No Hit
TGGAACACTCCTAGTA78460.15248876789814078No Hit
TGCAGATACTGCCACG77230.15009823533996192No Hit
CTTTAACTGTCCGTAA76700.14906816846529947No Hit
TGATAGAGAGACTGTT75280.14630836665016617No Hit
AGTCCTGCTCGAACGA73690.14321816602617887No Hit
CACATCCTGAGCCTAT73090.14205205258316478No Hit
AGGTGTGGATCATGTG72180.14028344719459343No Hit
TCTCAGAGATGCATGT71070.1381261373250174No Hit
TGCAGTATGACACCGC70840.13767912717186198No Hit
CTTAGATCTTGTCACA70300.13662962507314932No Hit
CATAGGTACAAGGTGC70160.13635753193644604No Hit
CTATTGGGACTTGTGA69860.135774475214939No Hit
TACGTTTACTGCACCG69760.13558012297443667No Hit
ATTTCTGACGGGTAGT68630.13338394265676015No Hit
TGGCAATTGAGTGGTG67890.1319457360770428No Hit
TCAAAGCACTCTGCAA66470.12918593426190947No Hit
TGAGGGATGTACGTTG64890.1261151688619724No Hit
TGAACTGTGAACGTAA64770.12588194617336956No Hit
AGAGTACCTTAATCGC64630.1256098530366663No Hit
CGAGTCTCTGCTACGC64550.12545437124426442No Hit
ATGTACGTGTACTTTC64340.1250462315392095No Hit
TCTATGGGATGCTCAA64180.12473526795440575No Hit
GCAACTTGAAGGTGCA63560.1235302840632912No Hit
TTCTTAGACGTCCCGA63370.12316101480633673No Hit
TACTGTTTGCTTAAGC60230.11705835445456308No Hit
TCCATTTGAAATCTGC60210.1170194840064626No Hit
CTACAACGAATCTGTG60050.11670852042165886No Hit
GGTCTAAACTCCTACC59730.11608659325205134No Hit
TTGCGGTCTTTTGGGT58610.11390984815842506No Hit
CTCAATTTGGAATCTC57810.11235503023440628No Hit
GGGTGTGCTCGCAAAG57730.11219954844200442No Hit
GAGTGTTACACCTGCG57290.1113443985837941No Hit
CTAGCTCACGAATAGT57290.1113443985837941No Hit
TCGGACCGATAGGCGA57140.11105287022304057No Hit
CACCAGGTGCGCCACA57080.11093625887873917No Hit
CTTTCGTCTCGAACGA56850.11048924872558377No Hit
CGAGTCTCTTGGAACG55910.10866233766486172No Hit
CATTCTATGCCTAGCG55900.1086429024408115No Hit
GGGATGTGACTGGAAG55500.10786549347880209No Hit
AGCCAATTGTTATGGC55420.10771001168640024No Hit
TGCTAGTGAAGGCGTA55130.10714639018894341No Hit
TCCGGGTGAACACCTA55030.10695203794844108No Hit
TGTAAGCACGCGCTGT54870.10664107436363732No Hit
ACCAAGTTGAAGCTAA54710.10633011077883356No Hit
GGTCGCATGGTACTAC54620.10615519376238144No Hit
CACATTACTGATGGAC54510.10594140629782886No Hit
TGCGCAGACATCAGGG54150.10524173823202043No Hit
AGGCGATGACCTGCGA53910.1047752928548148No Hit
TAGTACCGAACGAGCA53650.10426997702950869No Hit
ACCTCAGCTAATCCGT53640.10425054180545847No Hit
GTAATCTCTACACAAA53360.10370635553205189No Hit
CATAGTCGAAGATTCC53140.10327878060294672No Hit
ATGTCCAGACCGCTAG53140.10327878060294672No Hit
AGACTACTGGCCCTAG53080.10316216925864531No Hit
CTAACAGTGCATGATC52120.10129638774982279No Hit
AGCTTGTGATTGCGTA51950.10096598894096881No Hit
ATAGCCAACCGTTCTC51560.10020801520300966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)