FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004948521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17592679
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1880491.0689048552525742No Hit
TGCTGCTACCGTTCTC1811061.029439575405201No Hit
AAACGACCTCTTCAAG1767371.0046053815908311No Hit
ATGGGTTACAATCGAA1714510.9745587923249212No Hit
TATGCTTTGGACTGTT1699750.9661689387955068No Hit
GCAGGTCTGGCTGTTA1573440.8943720282738065No Hit
AAAGCGGACTCGGGCT1566220.8902680484308274No Hit
TCCAAAGTGCTCACGG1493760.8490804612532293No Hit
TAGTGCATGAACCCGC1418310.8061933034758378No Hit
GAAGTATGAACAGGTG1384730.78710581827816No Hit
TGTGGCTCTTACGATC1331610.7569114402644418No Hit
TAACTCACTGGTGTCC1325930.7536828245430954No Hit
TTCCCATCTCTTTGTT1199060.6815675997953466No Hit
TCACCTGACTCAATGC1137350.6464905089213531No Hit
GCCTCTTGATCTGTGA1117490.6352017222618568No Hit
GGAACTACTACCAAGG1108130.6298813273407649No Hit
TGAGAATGAGCAGTTT1105950.6286421755322199No Hit
ATGTCCAGAGTCGGTC1055150.5997665278835589No Hit
GAAGTAATGAGTCTGT1052340.5981692725707097No Hit
CTAGAGACTAATCTGC1043340.5930535082235059No Hit
TCGTATACTGCTAATC1026860.583685975285515No Hit
TTCGTCCGACCTGTGG1026190.5833051350507789No Hit
TCACACAGATTGGGAG1021660.5807302003293529No Hit
TGCGGTGACCTTAGTG1016630.5778710564775269No Hit
ATTCCTAACTGCTACA990370.5629443929489079No Hit
CCATGATGATTCGGGC987550.5613414534534508No Hit
AGGTCGAGAAGGGAGG973670.553451807993541No Hit
TCCGCGAGAGAACAGC971780.5523774974806281No Hit
ACTGATTCTCGGCTCA957650.5443457474555183No Hit
TGGACCTCTAGGGAGG937540.5329148562308219No Hit
GCAATCATGGAACGTG911560.5181473498152271No Hit
TGGCCTGGACCCGAGT886130.5036924734430726No Hit
TTCGATGGAAATCCTG824160.46846759382127073No Hit
ACGAGGATGGAGCTGA804300.4571788071617745No Hit
AAGGTGCGAACAGATA782560.44482139417197347No Hit
TTTCGTAACAGTACGG776290.44125741167675486No Hit
TCCCAGACTGTCTTGA753460.4282804227826813No Hit
TGGTCCTTGGAGAGGG752810.42791095091316106No Hit
TCTCTCTACTCTGGAT736680.41874236436645035No Hit
GCGGTTCGAGTACGTT727080.4132855490627664No Hit
TCCTTCACTTGTCACA655530.3726152225024966No Hit
TTCGATGGACAGGTGA647260.3679144034856772No Hit
TCGCCGAGAAGGTAAT646530.3674994581552929No Hit
TGATATCCTCGATGTT640840.36426515825133854No Hit
AGCTGCTCTTGTCACA636410.3617470653559927No Hit
AACTCCCTGCTTCACC623120.35419278666995513No Hit
CTTTCGGTGCTTTGTT616590.35048101542692844No Hit
AGCCCAGTGAAAGTAC588140.33430951590715663No Hit
AACCCTCACGCGGTTC572910.32565250579516625No Hit
TCGGCTTACCTCCTAC568760.32329357001284453No Hit
CCCTTGCCTCCTACGG562840.3199285339089061No Hit
ATGGCCGTGATACGCT560190.318422225517785No Hit
TGTTTGTTGCAAGTTC541830.3079860662494893No Hit
AAACACGCTGCAGTTT538870.3063035481975201No Hit
GGGGGGGGGGGGGGGG532110.3024610407545093No Hit
CTAACTGGAACCTTGA520300.2957480210944564No Hit
TACAACGTGAGCAGGT508150.2888417392257313No Hit
CAATGTGTGGTTGACT506210.2877390077997785No Hit
TGGCCTTTGCGAAAGC500970.2847604961131843No Hit
TCAAAGGCTCTTAGTG496340.2821287195656784No Hit
AAATAGGACTCTATCT492070.27970157359206066No Hit
ACAGCCGACAAACATG483160.27463696688832895No Hit
GGACATTTGGCCGCTA468120.26608795624589066No Hit
ACCCTTGTGGGCCATC467280.2656104849068183No Hit
CTTTCGGTGAGCCATT466650.26525238140251406No Hit
TGAACTGTGTGCTGGC458720.26074482459436676No Hit
AGTGTTCGATTCGCCT438540.24927414409141438No Hit
CAGCCGATGTAATAGG432020.24556805703099568No Hit
GAGGTTGGAGGAATAT428770.2437206976833943No Hit
GATAAGAACATTGGTC404780.23008434360679234No Hit
GGCTTGCGAATACGCT399270.22695235898978205No Hit
GTGCTAATGGGAGTCA387640.2203416546166732No Hit
CGACGTTCTTGCTACA377780.2147370505651811No Hit
GTAAAGCCTGAAATCA371630.21124127826125855No Hit
TTGGGAGGAATCTGTG369400.20997370553967362No Hit
TGTACTGGAAATGCTC361580.2055286747402144No Hit
GGAACATCTTTACGAC349500.19866218214974535No Hit
AGGGACCCTAATCCTG340550.19357483871558162No Hit
GGGTGAAACCGACTAT335820.19088622034199568No Hit
TCTTGAACTCGTACAT334150.18993696184645897No Hit
TAACGAGCTTACTTTC328650.18681066141205668No Hit
TACGTGTGAAACGCAG327260.18602056002954412No Hit
CGGATGAGAACAGGCA325000.18473593476013517No Hit
TACCACCTGAAACATG323840.18407656957760668No Hit
AGCTTGTGAACCGTGC314890.17898922614344295No Hit
ACTTATCTGTCAGGTT308640.17543661201344035No Hit
CATGATGTGCCTAGTA305960.17391325107449523No Hit
AACTAGCACTGCCACG299570.1702810583879806No Hit
TGGTAGGGATGTGCTA280310.15933332268496458No Hit
ACGTGTTGATCTTTGT279330.15877627278938017No Hit
TCGGACCGAATGTCCA279150.1586739575024361No Hit
AACTACGACATTGCCA272630.1549678704420174No Hit
CGTCGTACTTGCGCCA271180.1541436639638568No Hit
GTAGATAGAGCTGAGA269280.153063669268336No Hit
AACGAACCTGACACCC269180.15300682744225594No Hit
CTCGGATCTAGTACGG263120.14956221278180543No Hit
GGTAGTTTGTCCAGCG261340.1485504282775807No Hit
TTTAGGGACTGGGCAA261160.14844811299063662No Hit
CCTTCAGTGCAACGCC258670.1470327515212436No Hit
AGTTTGCGAGCAGGAT255000.14494665650410604No Hit
TACCACCTGTACTTTC252060.14327550681735285No Hit
TGAGGAGTGGTCCCAG249910.14205340755663196No Hit
CTTTAACTGTTCGCCT242530.13785848079192486No Hit
CAGGCCATGTCGTAGT235020.13358965965331374No Hit
AGAACGCGACACAGTT234300.13318039850553745No Hit
CTTACATTGGAAGGCT230000.13073619998409564No Hit
GAAAGTCCTTATCTGG225880.12839431674959795No Hit
GAGAAGAGATGCTACA225660.12826926473222186No Hit
GCCATAATGACCGTTA224460.12758716281926136No Hit
GGGATGTGATGCATGT223480.12703011292367694No Hit
CGATCGGTGCGAGGAT221030.12563748818471593No Hit
CAAAGACCTGCTTTGA220190.12516001684564357No Hit
TGCAATGACTCCGTAA218480.12418802161967488No Hit
GAAGGACACCCAGCTG214480.12191434857647321No Hit
GCTGGACACATTGGTC211420.12017498869842393No Hit
AACTACGACGCTGTTA210320.11954972861154348No Hit
AATTCTGCTTAGCGAG210290.11953267606371945No Hit
CCGTTACTGTTGGTTG208200.1183446818986466No Hit
TCCTCATCTTTCTGGA204710.11636090216845314No Hit
GGCTTAAACTGCATCA197390.1122000804993941No Hit
TGTCTTCCTGAAATCA196710.1118135560820498No Hit
TCGTAGAACCGCACTG195980.1113986107516655No Hit
GGACCACTGGCTAGAG194310.11044935225612881No Hit
CCCACCTCTTGACCTA192110.1091988320823679No Hit
ATGGCCGTGGGCCAAA191990.10913062189107185No Hit
GCGTTTAGAAGTCTAC191610.1089146229519677No Hit
ATAGCCAACATCTGTG190330.10818704757814315No Hit
TCCGTAAGATTACGCA189970.107982417004255No Hit
ACAAGACCTAAGCTAA187910.10681147538700614No Hit
TATGGGTACCCTTTGC184930.10511758896982092No Hit
CCTGAGGACTGACTCA184730.10500390531766084No Hit
AGGTGATCTGTTAGTC181890.10338959745698766No Hit
GCGTTAATGGTACGTT180150.10240054968319492No Hit
GGCTTCTACATCAGCT176340.10023487610954535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)