FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30810208
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404630.13132985015875256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG36550.08.460855
CTAGGAC49050.08.2420323
TACACTG56200.08.16675
GTATAGG28300.08.1577521
TAGACTG42800.08.1407555
GTACCGA9100.07.9975686
TAGACAG52800.07.97703845
GTCTTAG37500.07.9473331
TCTATAC26850.07.86698253
GTGTATA54700.07.8527921
TAGGACT45000.07.67933374
TACACCG12850.07.62261535
CTACACT53450.07.48613174
ATACTGT48250.07.48373466
GTCCTAG46550.07.4542681
TCTACAC44300.07.4206113
GTCCTAA32100.07.40997841
GTACTAG20050.07.39715151
CTATACT36400.07.2643964
GTTCTAG41650.07.2562421
Produced by FastQC (version 0.11.5)