FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004948534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3920641
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT402601.026872901650521No Hit
AAACGACCTCTTCAAG392301.0006016873261285No Hit
TGCTGCTACCGTTCTC391910.9996069520264671No Hit
ATGGGTTACAATCGAA380800.9712697490027778No Hit
TATGCTTTGGACTGTT374140.9542827308085591No Hit
AAAGCGGACTCGGGCT343430.8759537024685505No Hit
GCCTCTTGATCTGTGA331900.8465452460452259No Hit
TCCAAAGTGCTCACGG329120.8394545687809722No Hit
GCAGGTCTGGCTGTTA328990.8391229903477518No Hit
TAGTGCATGAACCCGC308760.7875242849319792No Hit
TAACTCACTGGTGTCC290700.7414603887476563No Hit
TGTGGCTCTTACGATC290300.7404401474146701No Hit
GAAGTATGAACAGGTG284180.7248304550199827No Hit
TTCCCATCTCTTTGTT263970.6732827616708594No Hit
TCACCTGACTCAATGC250910.6399718821488629No Hit
TGAGAATGAGCAGTTT245240.6255099612537848No Hit
GGAACTACTACCAAGG240940.6145423669241841No Hit
ATGTCCAGAGTCGGTC232000.5917399731319445No Hit
TCGTATACTGCTAATC229250.584725813967665No Hit
CTAGAGACTAATCTGC228110.5818181261686545No Hit
GAGCCTGACCGAGTTT227980.5814865477354341No Hit
TTCGTCCGACCTGTGG227940.5813845236021356No Hit
TCACACAGATTGGGAG227180.5794460650694618No Hit
ATTCCTAACTGCTACA221890.5659533734407206No Hit
GAAGTAATGAGTCTGT221450.5648311079744358No Hit
TGCGGTGACCTTAGTG220970.5636068183748524No Hit
TCCGCGAGAGAACAGC217360.5543991403446529No Hit
AGGTCGAGAAGGGAGG213950.5457015829809463No Hit
ACTGATTCTCGGCTCA210950.5380497729835504No Hit
TGGACCTCTAGGGAGG207510.5292756975198699No Hit
TGGCCTGGACCCGAGT203250.5184101273235677No Hit
CCATGATGATTCGGGC200450.511268437992665No Hit
GCAATCATGGAACGTG185270.4725502794058421No Hit
TTCGATGGAAATCCTG182180.4646689151085243No Hit
ACGAGGATGGAGCTGA181100.4619142635094618No Hit
AAGGTGCGAACAGATA173320.44207056958288193No Hit
TTTCGTAACAGTACGG170320.43441875958548615No Hit
TCCCAGACTGTCTTGA169580.43253131311946186No Hit
TGGTCCTTGGAGAGGG166110.4236807195558074No Hit
TCTCTCTACTCTGGAT162210.4137333665591927No Hit
TCGCCGAGAAGGTAAT144020.36733789194164934No Hit
TTCGATGGACAGGTGA143780.3667257471418577No Hit
AGCTGCTCTTGTCACA142720.3640221076094445No Hit
TGATATCCTCGATGTT142700.36397109554279516No Hit
TCCTTCACTTGTCACA141500.36091037154383687No Hit
GCGGTTCGAGTACGTT138430.35308001931316846No Hit
AGCCCAGTGAAAGTAC137080.34963670481434034No Hit
AACTCCCTGCTTCACC136100.34713711354852433No Hit
CTTTCGGTGCTTTGTT135580.3458107998156424No Hit
AACCCTCACGCGGTTC131390.3351237718526129No Hit
CCCTTGCCTCCTACGG128440.3275994920218403No Hit
ATGGCCGTGATACGCT128260.3271403834219966No Hit
TCGGCTTACCTCCTAC124260.3169379700921354No Hit
TGTTTGTTGCAAGTTC118920.30331774829677083No Hit
AAACACGCTGCAGTTT117470.2996193734646962No Hit
GGGGGGGGGGGGGGGG116880.2981145174985417No Hit
ACCCTTGTGGGCCATC114710.29257970826709206No Hit
TGGCCTTTGCGAAAGC114540.2921461057005729No Hit
CTAACTGGAACCTTGA113050.28834570673519966No Hit
CAATGTGTGGTTGACT111010.2831424759369705No Hit
TACAACGTGAGCAGGT110150.2809489570710504No Hit
AAATAGGACTCTATCT108460.27663843743918404No Hit
TCAAAGGCTCTTAGTG106670.2720728574740712No Hit
ACAGCCGACAAACATG104450.2664105180759983No Hit
CTTTCGGTGAGCCATT102390.2611562752111198No Hit
TGAACTGTGTGCTGGC101820.25970243131161463No Hit
CAGCCGATGTAATAGG96170.2452915224831858No Hit
AGTGTTCGATTCGCCT96050.24498545008328995No Hit
GGACATTTGGCCGCTA94140.24011379771828129No Hit
GAGGTTGGAGGAATAT87450.22305026142408857No Hit
CGACGTTCTTGCTACA82510.21045028096171012No Hit
TGTACTGGAAATGCTC80440.20517053206350697No Hit
TTGGGAGGAATCTGTG80070.20422680883049482No Hit
GTGCTAATGGGAGTCA78200.19945718059878473No Hit
AGGGACCCTAATCCTG74680.19047905686850697No Hit
GGAACATCTTTACGAC74600.19027500860190974No Hit
TAACGAGCTTACTTTC73280.1869082122030556No Hit
TCTTGAACTCGTACAT73100.18644910360321182No Hit
GGGTGAAACCGACTAT73070.18637258550323785No Hit
GGACCACTGGCTAGAG72990.18616853723664065No Hit
TACCACCTGAAACATG72770.18560740450349827No Hit
TACGTGTGAAACGCAG72530.18499525970370662No Hit
CGGATGAGAACAGGCA71260.1817559934714757No Hit
AGCTTGTGAACCGTGC67970.17336450850766494No Hit
ACTTATCTGTCAGGTT67030.1709669413751476No Hit
CATGATGTGCCTAGTA66730.17020176037540802No Hit
ACGTGTTGATCTTTGT62840.16027991341211809No Hit
TCGGACCGAATGTCCA61940.1579843704128993No Hit
AACTAGCACTGCCACG61740.15747424974640625No Hit
CGTCGTACTTGCGCCA60950.1554592731137587No Hit
TGGTAGGGATGTGCTA60590.1545410559140712No Hit
AACTACGACATTGCCA60180.15349530854776042No Hit
CTCGGATCTAGTACGG58830.1500519940489323No Hit
GTAGATAGAGCTGAGA58140.14829207774953126No Hit
AACGAACCTGACACCC57430.14648114938348092No Hit
TACCACCTGTACTTTC56500.1441090882842882No Hit
GGTAGTTTGTCCAGCG56460.1440070641509896No Hit
TTTAGGGACTGGGCAA56200.14334390728454863No Hit
AGTTTGCGAGCAGGAT56120.1431398590179514No Hit
CCTTCAGTGCAACGCC55430.14137994271855037No Hit
TGAGGAGTGGTCCCAG54760.13967103848579862No Hit
GTCCGAGTGGTCTTGA54650.13939047211922745No Hit
GGCTTGCGAATACGCT53740.13706942308668404No Hit
GCCATAATGACCGTTA53540.136559302420191No Hit
CTTTAACTGTTCGCCT53310.13597266365372396No Hit
AGAACGCGACACAGTT51430.13117752938868926No Hit
CTTACATTGGAAGGCT50400.12855040795625No Hit
GTCCGTTACGGATGGT50070.12770870885653648No Hit
CAGGCCATGTCGTAGT49760.12691802182347223No Hit
TGCAATGACTCCGTAA49540.12635688909032988No Hit
GGGATGTGATGCATGT48750.1243419124576823No Hit
CCGTTACTGTTGGTTG48590.12393381592448785No Hit
CAAAGACCTGCTTTGA48560.1238572978245139No Hit
GAGAAGAGATGCTACA47450.12102612812547744No Hit
CGATCGGTGCGAGGAT47420.12094961002550347No Hit
GAACCATACTTTGGGT47170.12031195919238716No Hit
AATTCTGCTTAGCGAG46710.11913868165945314No Hit
AACTACGACGCTGTTA45990.11730224726007814No Hit
TCCTCATCTTTCTGGA45170.1152107525274566No Hit
GAAAGTCCTTATCTGG43740.11156338976203127No Hit
TGTCTTCCTGAAATCA43580.1111552932288368No Hit
CCCACCTCTTGACCTA43510.11097675099556424No Hit
TCGTAGAACCGCACTG43200.11018606396250002No Hit
TCCGTAAGATTACGCA42750.10903829246289064No Hit
ATGGCCGTGGGCCAAA42710.10893626832959202No Hit
GATAAGAACATTGGTC42300.10789052096328126No Hit
GAAGGACACCCAGCTG41540.10595206243060765No Hit
CCTGAGGACTGACTCA41270.10526339953084202No Hit
TATGGGTACCCTTTGC40760.10396259183128474No Hit
AGGTGATCTGTTAGTC40000.10202413329861112No Hit
ACAAGACCTAAGCTAA39840.10161603676541668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)