FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15726125
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157710.10028535319412761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA34000.010.1817891
TATACCG2451.1610609E-89.71351055
GTCTTAG19100.09.0990151
TAGGACA20900.08.5070394
ATACCGT3951.2732926E-118.5054616
TACACCG6300.08.4437715
GTATAAG28200.08.4404663
TATACTG16300.08.1588585
TCTATAC12900.08.1402583
GTTCTAG20150.08.1379851
TGTATAA33800.08.1211822
GCACCGT12600.07.99918376
TACACTG25200.07.94381145
TAGACTG21000.07.9327015
CCTACAC21950.07.7820123
TAGACAG28500.07.71166565
GTCTTAC15450.07.7107481
CTAGGAC22400.07.6881833
GGACCGT8150.07.55751136
CTAGTAC8950.07.50904663
Produced by FastQC (version 0.11.5)